Options And Accessories; Column Heater; Column Heater/Cooler; Column Pre-Heater - Waters Alliance e2695 Operator's Manual

Separations module
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1.5

Options and accessories

Various options for the separations module are available to suit your applications and site
requirements. You can display a list of the hardware options currently installed in your separations
module by pressing the Configuration screen's Options key. See
module" on page 47
1.5.1

Column heater

The column heater maintains the column at temperatures from 5 °C above ambient (minimum of
20 °C) to 60 °C. An alarm warns you when the temperature varies outside the range that you
specify. The Waters Alliance Series Column Heater and Column Heater/Cooler Operator's Guide
presents the procedures for installing, operating, maintaining, and basic troubleshooting of the
Alliance column heaters and column heater/coolers.
1.5.2

Column heater/cooler

The column heater/cooler expands the heating range over the column heater, while offering
column cooling capabilities. The column heater/cooler maintains the column at temperatures from
4 °C to 65 °C, with a set point temperature range that is ambient minus 15 °C or 4 °C (whichever is
greater) to 65 °C, in 1 °C increments. The Waters Alliance Series Column Heater and Column
Heater/Cooler Operator's Guide presents the procedures for installing, operating, maintaining, and
basic troubleshooting of the Alliance column heaters and column heater/coolers.
1.5.3

Column pre-heater

For certain applications, especially in laboratories with less than ideal temperature control, the
column pre-heater is useful in stabilizing the temperature of the mobile phase as it enters the
chromatographic column. This stabilization, or conditioning, of the mobile phase improves
retention-time precision and overall chromatographic performance. The Pre-Heater Plate
Assembly (WAT271064) does not come as standard equipment in either the column heater or the
column heater/cooler; it is an option that must be ordered separately and installed at the e2695
Alliance instrument site.
1.5.4

Sample heater/cooler

To optimize sample stability and/or solubility, the sample heater/cooler maintains the sample
compartment at temperatures from 4 °C to 40 °C, with a set point temperature range that is
ambient minus 25 °C or 4 °C (whichever is greater) to 40 °C, in 1 °C increments. This option is
installed on the separations module at the factory or is available as an option installed on site by
Waters personnel. The heater/cooler mounts through the rear panel of the separations module. It
uses four Peltier devices for temperature control.
1.5.5

Syringes

You can install a 25-µL, 100-µL, 250-µL, or 2500-µL sample syringe in the separations module.
The 250-µL syringe is standard. (See
replace the syringe.)
for details on displaying the Configure screen.
"Replace the syringe" on page 137
February 27, 2018, 715003794 Rev. C
Page 33
"Configure the separations
for the procedure to

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