Troubleshooting Hardware Problems - Waters Alliance e2695 Operator's Manual

Table 8–9: Chromatography troubleshooting (continued)
Symptom
Sensitivity loss
Sample energy decreased,
reference energy not
decreased
8.5.4

Troubleshooting hardware problems

This section contains troubleshooting tables that address these issues:
• System problems
• Solvent management system problems
• Sample management system problems
Before you use these tables, read
cause of the hardware symptom.
Possible cause
Leak in sample management
system
Degraded, contaminated, or
improperly prepared sample
Column contaminated
Loss of column efficiency
Peaks wider than expected
Incorrect detector filter time
constant
Change in mobile phase
composition
Leak in flow cell
Incorrect flow rate
Contaminated mobile phase
Dirty flow cell
"General troubleshooting hints" on page
February 27, 2018, 715003794 Rev. C
Page 175
Corrective action
Troubleshoot sample
management system.
Use fresh sample.
Clean/replace column.
Clean/replace column.
Troubleshoot sample
management system. Inspect
fittings for leaks and proper
ferrule setting. Confirm tubing
inner diameter is correct.
Set correct detector filter time
constant.
Correct mobile phase pH or
ionic composition.
Tighten cell seal.
Change flow rate.
Use fresh mobile phase.
Clean outside of flow cell
windows.
Flush system with water. If
necessary, remove the
column, and then flush the
system with stronger solvent
to remove particulate or filmy
matter from flow cell windows.
169, and isolate the