Prime The Plunger Seal-Wash Pump - Waters Alliance e2695 Operator's Manual

3 Prepare the e2695 Separations Module for Operation
• The Pressure field displays the current vacuum level in psia, bar, or
kPa.

Prime the plunger seal-wash pump

The seal-wash solvent lubricates each plunger and flushes away any solvent
or dried salts forced past the plunger seal from the high-pressure side of the
piston chamber. This wash cycle extends the life of the seals. Position the
plunger seal-wash reservoir in a visible location above the solvent
management system, and refill the reservoir as necessary.
Caution:
wash and needle wash. Because the functions of these solutions differ,
using one solution for both functions can compromise the effectiveness of
either needle washing or plunger-seal washing.
For reversed-phase HPLC applications, use an aqueous plunger seal-wash
solution with enough organic content to inhibit bacterial growth. For example,
use an 80% water/20% methanol solution or an 80% water/20% acetonitrile
solution, depending on your application. For GPC seal wash solvent
recommendations, see
To prime the plunger seal-wash pump for first-time use or when the entire
line is dry:
If you are adding the same plunger seal wash-solution or changing to a
Tip:
solution that is totally miscible with the existing solution, perform only
step 5, step
1. Press the Diag screen key in the Main screen.
Result:
tests" on page 221
2. Attach the syringe adapter to the syringe (both are in the startup kit).
3. Remove the inlet filter from the seal-wash inlet tube.
4. Fill the syringe with the seal-wash solution, and then attach the syringe
adapter to the end of the seal-wash inlet tube.
5. Press the Prime Seal-Wash screen key, and then press the Start screen
key.
106 July 29, 2013, 715003794 Rev. B
Use separate solutions and containers for the plunger-seal
"GPC solvents and seal choices" on page
6, and step 8.
The Diagnostics screen appears. See
for more information on the Diagnostics screen.
293.
"Perform main diagnostic
step 1,