Prime The Plunger Seal Wash Pump; Prime The Solvent Management System - Waters Alliance e2695 Operator's Manual

3.4.3

Prime the plunger seal wash pump

The seal wash solvent lubricates each plunger and flushes away any solvent or dried salts forced
past the plunger seal from the high-pressure side of the piston chamber. This wash cycle extends
the life of the seals. Position the plunger seal wash reservoir in a visible location above the solvent
management system, and refill the reservoir as necessary.
Notice: Use separate solutions and containers for the plunger-seal wash and needle wash.
Because the functions of these solutions differ, using one solution for both functions can
compromise the effectiveness of either needle washing or plunger-seal washing.
For reversed-phase HPLC applications, use an aqueous plunger seal wash solution with enough
organic content to inhibit bacterial growth. For example, use an 80% water/20% methanol solution
or an 80% water/20% acetonitrile solution, depending on your application. For GPC seal wash
solvent recommendations, see
To prime the plunger seal wash pump for first-time use or when the entire line is dry:
Tip: If you are adding the same plunger seal wash-solution or changing to a solution that is totally
miscible with the existing solution, perform only
not used, steps 2 and 4 can be skipped.
1. Press the Diag screen key in the Main screen.
Result: The Diagnostics screen appears. See
for more information on the Diagnostics screen.
2. Attach the syringe adapter to the syringe (both are in the start-up kit).
3. Press the Prime Seal-Wash screen key, and then press the Start screen key.
4. Attach the syringe with adapter to the seal wash waste line and pull.
5. When seal wash solvent flows out the seal wash waste tube, press the Halt screen key.
6. Reinstall the inlet filter, and place the seal wash inlet tube in the seal wash reservoir.
7. Press Close to return to the Diagnostics screen.
3.4.4

Prime the solvent management system

Prime the solvent management system using one of these priming methods:
• Dry prime, when the solvent lines are dry
• Wet prime, when you want to change between miscible solvents
Follow the procedure in
as appropriate.
Once you prepared the solvent management system for operation, you must prepare the sample
management system for operation.
"GPC solvents and seal choices" on page
step
"Dry prime the system" on page 74
February 27, 2018, 715003794 Rev. C
Page 58
224.
1, step 5, and step 6. If the syringe adapter is
"Perform main diagnostic tests" on page 150
or "Wet prime the system" on page 76,