Sample Management Specifications - Waters Alliance e2695 Operator's Manual

C.5

Sample management specifications

The following table lists the sample management specifications for the Alliance e2695 Separations
Module.
Table C–5: Sample management specifications
Item
Number of sample vials
Number of sample injections 1 to 99 injections per sample vial
Sample delivery precision
Injector needle wash
Sample carryover
Injection accuracy
Standard sample vial
Sample temperature control
(optional)
Advanced operations
Injection volume range
Injector linearity
a.
Specification
120 vials configured in 5 carousels of 24 vials each
Typically < 0.5% RSD, 5 to 80 µL
(Degassed methanol/water 60:40 Dial-a-Mix, 1 mL/min,
six replicates, phenone mix, 254 nm)
Integral, active, programmable
≤ 0.0025% (based on the following chromatographic conditions):
1
• Column – XBridge C18, 4.6 x 50 mm, 3.5 µm
• Mobile phase – 70% water/30% methanol
• Needle wash – 100% Methanol
• Challenge sample – 4.0 mg/mL caffeine (in mobile phase)
• Carryover standard – 0.4 mg/mL caffeine (in mobile phase)
• Injection volume – 10 µL
±1 µL (±2%), 50 µL, N = 6
Sample is degassed water, analytical solvent is degassed
methanol
2 mL
Ambient minus 25 °C or 4 °C (whichever is greater) to 40 °C,
in 1 °C increments
• ± 3 °C temperature accuracy
• 60-minute time limit from lab ambient to heating set-point
• 90-minute time limit from lab ambient to cooling set-point
Stat runs, auto additions, auto standards
0.1 to 100 µL, standard
0.1 to 2000 µL, with optional sample loop
> 0.999 coefficient of deviation (1 to 100 µL)
February 27, 2018, 715003794 Rev. C
Page 221