Solvent Compatibility - Waters Alliance e2695 Operator's Manual

D Solvent Considerations
Recommended plunger seal and seal wash solution to be used for GPC
solvents: (Continued)
GPC Solvent
Dimethylacetamide
c
(DMAC)
Hexafluoroisopro-
panol (HFIP)
Methylene chloride
N,N-dimethylform-
amide (DMF)
N-methyl-2-pyrroli-
done (NMP)
Tetrahydrofuran
(THF)
e
Water
a. Package contains 2 seals, P/N: WAT270938.
b. Package contains 2 seals, P/N: 700001326.
c. This solvent is typically used with an additive of 0.05M lithium chloride or lithium bromide.
d. HFIP is typically used with an additive of 0.05M sodium trifluoroactetate.
e. Most aqueous GPC separations use a salt or buffer.
To prevent premature plunger seal failure when using toluene,
o-dichlorobenzene (o-DCB), or 1,2,4 trichlorobenzene (TCB), Waters
recommends the use of the optional Alliance Clear Seals, the removal of
installed seal wash seals, and no seal wash solvent. The use of graphite-filled
PTFE (black) seals is not recommended for GPC applications with the e2695
Separations Module.

Solvent compatibility

The separations module is constructed of high-quality (316) stainless steel
components that, with some minor restrictions, can be used with all solvents.
294 July 29, 2013, 715003794 Rev. B
With standard
a
seal
Fluoroloy G
(yellow)
Do not use
Recommended
d
Recommended
Do not use
c
Do not use
c
Strongly
recommended
Recommended
With optional
b
seal
Seal wash
Alliance Clear
solution
Seal
(opaque)
Strongly 100% methanol
recommended
Strongly 50:50
recommended methanol/water
Recommended 100% methanol
Strongly 50:50
recommended methanol/water
Strongly 50:50
recommended methanol/water
Strongly 50:50
recommended methanol/water
Recommended 20:80
methanol/water