Prepare A New Or Dry Separations Module For Operation; Prepare An Idle Or Powered-Off Separations Module For Operation - Waters Alliance e2695 Operator's Manual

3.6.1

Prepare a new or dry separations module for operation

To change from a buffered solvent to a totally organic solvent:
1. Prime the plunger seal wash pump (see
2. Prime the needle wash pump (see
3. Fill all four solvent reservoirs with fresh solvent filtered and degassed by vacuum filtration
(see "Prepare solvent reservoirs" on page
Tip: To maximize degasser efficiency (and fill all four degasser chambers with solvent), fill
all four solvent reservoirs (even if you use fewer than four solvents). If you choose water as
one of the "unused" solvents, change the water weekly to prevent bacterial contamination.
4. Set the degasser to On (see
77).
5. Perform a dry prime to fill each solvent line and degasser chamber (see
system" on page
6. Equilibrate the solvent in the degasser chamber by setting a flow rate of 0.000 mL/min for 5
min (see
7. Run System Prep (see
3.6.2 Prepare an idle or powered-off separations module for
operation
Notice: If you just performed the steps from the procedure, below, for changing from a
buffered solvent to an organic solvent, ensure that the Plunger Seal-Wash and
Needle-Wash solvents are compatible with the new solvents.
To prime, equilibrate, and purge the separations module that is powered-off or idle with no
solvent flow:
1. Check plunger seal wash fluid levels. Refill and prime, if necessary (see
seal wash pump" on page
2. Check needle wash fluid levels. Refill and prime, if necessary (see
pump" on page
3. Set the degasser to On (see
77).
4. Run System Prep (see
"Equilibrate solvents in the in-line vacuum degasser" on page
74).
"Equilibrate solvents in the in-line vacuum degasser" on page
"Run System Prep" on page
58).
60).
"Equilibrate solvents in the in-line vacuum degasser" on page
"Run System Prep" on page
February 27, 2018, 715003794 Rev. C
"Prime the plunger seal wash pump" on page
"Prime the needle wash pump" on page
57).
75).
75).
Page 63
58).
60).
"Dry prime the
77).
"Prime the plunger
"Prime the needle wash