Mixed Mobile Phases - Waters Alliance e2695 Operator's Manual

Table D–7: UV cut-off wavelengths for common chromatographic solvents (continued)
Solvent
Ethyl ether
Ethyl sulfide
Ethylene dichloride
D.8.2

Mixed mobile phases

The following table provides approximate wavelength cut-offs for some other solvents, buffers,
detergents, and mobile phases. The solvent concentrations represented are those most commonly
used. If you want to use a different concentration, you can determine approximate absorbance
using Beer's Law, since absorbance is proportional to concentration.
Table D–8: Wavelength cut-offs for different mobile phases
Mobile phase
Acetic acid, 1%
Ammonium acetate, 10 mM
Ammonium bicarbonate,
10 mM
BRIJ 35, 0.1%
CHAPS, 0.1%
Diammonium phosphate,
50 mM
EDTA, disodium, 1 mM
HEPES, 10 mM, pH 7.6
Hydrochloric acid, 0.1%
MES, 10 mM, pH 6.0
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
Sodium acetate, 10 mM
UV cut-off (nm)
220
290
230
UV cut-off (nm) Mobile phase
230
205
190
190
215
205
190
225
190
215
190
190
205
February 27, 2018, 715003794 Rev. C
Page 233
Solvent
Tetrahydrofuran
Toluene
Xylene
Sodium chloride, 1 M
Sodium citrate, 10 mM
Sodium dodecyl sulfate
Sodium formate, 10 mM
Triethyl amine, 1%
Trifluoracetic acid, 0.1%
TRIS HCl, 20 mM,
pH 7.0, pH 8.0
Triton X-100, 0.1%
Waters PIC Reagent A,
1 vial/liter
Waters PIC Reagent B-6,
1 vial/liter
Waters PIC Reagent B-6,
low UV, 1 vial/liter
Waters PIC Reagent D-4,
1 vial/liter
UV cut-off (nm)
230
285
290
UV cut-off (nm)
207
225
190
200
235
190
202, 212
240
200
225
190
190