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CombiFlash NextGen Systems
Section 5 Maintenance
Error/Symptom
No signal on any mass, or very weak
signal
Delayed signal during run.
Sample masses observed but weak, or
masses other than the analyte
observed.
5-16
Table 5-2 General PurIon Troubleshooting
Solution
•
Verify nitrogen pressure. Nitrogen is required to nebulize the
sample.
•
Check carrier solvent level, refill carrier solvent and re-prime the
carrier solvent pump. Carrier solvent is needed to bring sample to
the mass spectrometer.
•
Check that the priming valve is tight. A loose priming valve
prevents sample from getting to the mass spectrometer.
•
Verify the splitting valve is not leaking. This causes loss of carrier
solvent preventing sample from getting to the mass spectrometer.
•
Check for leaks around other fittings on the solvent interface.
•
Verify nitrogen pressure. Sample may drip through the cone and
still be detected later.
•
Check priming valve tightness. A slight leak will delay sample.
•
Verify the splitting valve is not leaking.
•
Check for leaks around other fittings on the solvent interface.
•
Check polarity to see if the sample ionizes under the opposite
polarity.
•
Try another ionization settings.
•
Compound fragments – use fragment mass or try another
ionization setting.
•
Compound forms adducts.
•
Compound fails to ionize using the selected probe. Some
compounds ionize better using APCI rather than ESI, for example.
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
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