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EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
Note
If equipped with solvent level sensing and the waste level
sensing tube is inserted correctly into the waste container, the
systems will automatically suspend operation before an over-
flow condition might exist. To prevent the run from being sus-
pended before completing the run, ensure that the container
will hold the expected waste volume.
7. If equipped with an ELSD, ensure that the ELSD option is
selected if desired. The system defaults to ELSD detection
parameters that are compatible with fluids typically used
by the selected column. If performing a normal phase sepa-
ration using a high boiling point solvent such as heptane or
toluene, you may need to review the ELSD parameters in
the M
E
ETHOD
DITOR
The system will automatically select or deselect use of the
ELSD based on the previous run.
Note
If you typically use normal phase solvents and the ELSD
P-trap drain fluid hasn't been replenished recently, you may
need to ensure the P-trap is full for maximum ELSD signal
strength. This can be done by lifting the drain line above the
instrument and placing 10 mL of isopropyl alcohol in the drain
line. Lift the line sufficiently that the fluid flows into the P-trap
drain. Then place into a waste container so that the excess fluid
can drain out of the trap. If you normally use higher boiling point
solvents such as water, the P-trap will typically remain full and
will not require replenishment.
Note
If you are using a silica flash column and your solvent exceeds
7.5 pH, the ELSD should not be used. Basic conditions can
dissolve silica. When the solvent with dissolved silica is evapo-
rated in the ELSD, silica can build up over a period of time and
eventually clog the ESLD, resulting in an expensive repair.
Sample build up in the spray chamber is less of a concern
since it is sent to the chamber in smaller quantities only during
the peak and is more easily cleaned from the chamber during
regular cleaning (see Section 5.6 ELSD Maintenance).
Mass Spectrometer – (PurIon systems only) click this button to
enable mass spectrometer detection.
PurIon Loading – (PurIon systems only) sets the amount of
sample sent to the mass spectrometer.
• L
reduces saturation of the mass spectrometer
OW
detector. This is the default.
CombiFlash NextGen Systems
Section 3 Operation
; otherwise the ELSD may be disabled.
3-5
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