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CombiFlash NextGen Systems
Section 3 Operation
Figure 3-15 Selecting ion settings
3-22
I
F
and enter your desired mass, it will compare
ON
INDER
the 10 most intense ions with a list of common adducts
or fragments to determine if your desired mass was
detected as one of the peaks. If it was, you can select the
peak for detection in your separation.
• The displayed spectrum will continue to collect mass
information until the window is closed or the display
cleared operation of the injection valve will clear the
current display.
• If you compound isn't detected, you can adjust the
ionization settings by selecting from the list (Figure
3-15). The factory selections include:
· R
for compounds that do not easily ionize.
OBUST
· T
works well for most compounds.
YPICAL
· F
for those compounds that are delicate or
RAGILE
easily fragment.
When one of the ion settings is selected, you can use the sug-
gested settings or edit the parameters to optimize the ionization
settings. Options on the M
• Factory Settings – Restores the R
F
ion settings to the factory default values.
RAGILE
• Probe – The probe nebulizes and ionizes the sample.
There is a choice of ESI (electrospray interface) or APCI
(atmospheric pressure chemical ionization) probes. The
software will change the labels on the control to reflect
the probe installed in the mass spectrometer.
• Gas Temp – The temperature of the nebulization gas
for the probe. Lower temperatures are used for more
delicate, heat-labile compounds. The temperature is set
to quickly evaporate the carrier solvent. (Note that
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
D
window include:
ETHOD
EVELOPMENT
, T
OBUST
, and
YPICAL
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