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CombiFlash NextGen Systems
Section 3 Operation
3-4
• Solid (Pause) – Select this option if you will place the
sample into a solid sample load cartridge, but have not
yet prepared the sample. (Column plan subscribers
must also specify the cartridge type and size).
When you click the R
a column equilibration and then wait while you prepare
the cartridge. After you have placed the cartridge on the
system, click O
K
• Liquid – Select this option if you have prepared a liquid
sample and plan to manually inject it into the injection
port or directly onto the column after column equili-
bration.
· If you inject using the Luer fitting on top of the
column mount, inject the sample, then inject 3 mL of
chase fluid to wash the sample completely onto the
column. Make sure the chase solution is strong
enough to prevent sample crashing, but not so strong
as to adversely affect the chromatography.
• None (on column) – Select this option if you have
pre-loaded the sample on the column.
· The system will skip column equilibration so that the
sample will not be flushed from the column before the
run.
5. Review or update the S
6. Review other M
INIMUM
• The system will report the estimated solvent volumes,
expected waste, collection tube usage, etc. on this
window. You can use this information to verify that there
will be enough solvent to complete the run, the waste
volume does not exceed the collection container's
capacity, and whether more tubes will be required
during the run.
• End of Run Hold – Select whether the run will undergo
automatic valve wash and air purging without waiting
for user input.
If enabled, this allows the user to extend
the run if needed to complete the separation before
proceeding to the post run processes. This setting
defaults to the selection used for the previous separation.
Note
The CombiFlash systems with solvent level sensing estimate
the solvent volumes by monitoring the solvent level in the con-
tainer and the known usage rate. The system continues to
refine this estimate when in operation.
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
button, the system will perform
UN
to continue with the run.
R
and S
TART
ACK
R
R
UN
EQUIREMENTS
T
.
TART
UBE
:
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