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5.7.7 Replacing Check Valves
Figure 5-13 Correct orientation of cartridge
5.7.8 PurIon Cone Cleaning
5.7.9 Capillary Inlet Cone
Removal
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
5. Check the flow rate delivery. If the flow rate delivery is still
incorrect, replace the check valves. The correct flow rate
depends on the back pressure of the carrier solvent. The
flow will be 0.5 mL/minute until the fluid interface reaches
operating pressure, then reduces the flow rate to
0.2 mL/minute under typical operating conditions for the
fluid interface. The operating mode can be determined by
listening to the pump operate. While running at
50 mL/min, the pump motor speed is continuous. Once
operating pressure is reached, the pump has a rapid refill
stroke approximately that occurs ~8 seconds.
1. Install new check valve cartridges (P/N: 250-0001-17) into
pump head housing making sure the ends of the cartridges,
with three small holes, are facing upwards towards the
outlet (Figure 5-13).
2. Tighten check valve holders finger tight, then with pliers
tighten an additional ¼ turn.
This error occurs if the capillary inlet to the PurIon vacuum
region is restricted, causing a higher vacuum reading than
normal. The PurIon S & L models compatible with the NextGen
systems have an internal valve that allows removal of the cap-
illary inlet without venting the vacuum system to allow easy
cleaning. Even with the easy cleaning capability, Teledyne ISCO
recommends keeping a spare capillary inlet cone assembly
(Teledyne ISCO P/N 25-0000-085) to allow rapid replacement to
minimize downtime while cleaning the plugged capillary.
1. Set the PurIon to standby.
2. Wait ~15 minutes for the cone to cool.
3. Remove the ion source assembly, section 5.7.6.
4. Wearing gloves (typical lab gloves are usually sufficient),
place your fingers on the top surface of the cone and turn
counterclockwise to unscrew the capillary inlet cone. Many
times this is sufficient to remove the capillary. If you are
unable to unscrew the part manually, use an adjustable
wrench on the flats of the cone to unscrew. The flats are not
large; they may be difficult to see while keeping a wrench
seated on them.
CombiFlash NextGen Systems
Section 5 Maintenance
5-13
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