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Figure 3-14 Files screen
3.5.11 PurIon Operation
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
The PurIon functions like another detector in the system. In
addition, operation of the Mass Detector has been integrated into
the PeakTrak control software. To use the PurIon, turn the
PurIon power O
(if not already on). When turned on, the PurIon
N
requires 30 minutes to achieve proper vacuum levels. A
countdown window will monitor progress. Separations can be
completed without the PurIon during this period.
Operate/Standby/Shutdown: When ready to use the PurIon,
select the MS drop down and select O
the gas flow, heaters, and detector voltage. When not in use,
select S
to turn of the detector voltage and heaters. The
TANDBY
standby mode will prolong the life of the detector when not in
use. The S
command is used to power down the turbo
HUTDOWN
pump in the PurIon in preparation for repair or repositioning of
the system. Moving the system without shutting down can cause
major damage to the PurIon. Turbo speed is monitored during
the shutdown process.
Method Development (for PurIon): Choose this command to
open the MS M
D
ETHOD
conditions for the compounds to be purified. This command is not
available from a remotely connected computer via a web browser.
• To use the M
ETHOD
minute amount of crude sample in a minimal amount of
suitable solvent. Dilute this by placing 1 drop in
~ 20 mL of solvent. Place the front panel inject valve
into the load injection and inject >20 µL using a
22 gauge blunt-tipped needle. Within a few seconds, the
mass spectra will be displayed.
• If your ion of interest isn't visible, it could be due to the
formation of adducts or fragmentation. If you select the
CombiFlash NextGen Systems
Section 3 Operation
. This will turn on
PERATE
window to verify ionization
EVELOPMENT
D
screen, dissolve a
EVELOPMENT
3-21
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