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CombiFlash NextGen Systems
Section 5 Maintenance
5.7.6 Check Valve Cleaning
Figure 5-12 Check valve holder
5-12
PurIon source sprayer. The most common location for a plug is
within the probe capillary. The occurrence of plugs can be
reduced by using a 0.45 µ syringe filter when injecting samples
for Method Development and Ionization Settings. To trouble-
shoot an overpressure error, complete the following:
• If the error occurs during a run, select C
W
PurIon. The purification can continue without
ITHOUT
the PurIon signal or peak detection, but will rely on any
other detector selected such as UV or ELSD. This allows
the run to be completed before troubleshooting the plug.
• Use the menu item MS > Manual control.
• Press the S
C
TART
• Watch the pressure on the ribbon gauge.
• Loosen the fitting at the source inlet. If the pressure
drops, then the source capillary is plugged.
• If there is still an error or the pressure remains high,
then the plug is between that point and the splitter
valve (or the valve itself). Continue to loosen fittings
going back the to the splitter valve until the error is
corrected.
If the check valves are allowed to dry out after using volatile
salts (e.g., ammonium acetate or ammonium formate), they may
stick and fail to function. Complete the following to clean the
check valves:
1. Remove the inlet and outlet lines from the check valve
holders (Figure 5-12), and then remove the check valve
holders. Pliers may be needed to remove the holders.
2. Remove check valves from pump head and place in a bea-
ker of methanol. Sonicate check valves for 15 to 20 min-
utes.
3. Reinstall check valves into pump head, making sure that
the ends of the check valves (with multiple holes) are fac-
ing up towards the outlet of the pump.
4. Reinstall check valve holders and tighten finger tight.
Then using pliers tighten an additional ¼ turn.
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
ONTINUE
P
button.
ARRIER
UMP
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