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CombiFlash NextGen Systems
Section 3 Operation
3.5.7 Tube Volume
3.5.8 Peak Detection
Options
UV or UV-Vis
Figure 3-7 Wavelength #1 Detection Options window
3-14
• A
- Collects all fluid during the time window. Detected
LL
peaks will still trigger tube advances.
• N
- Diverts all fluid to waste.
ONE
Tube Volume – Sets the capacity for collected fluids. This
volume can be the default maximum volume (Max option) for the
tube size as defined in the C
method-specific volume less than the default maximum capacity.
· This is useful for collecting fractions smaller than the
maximum tube volume when the peak can't be
detected and must be located after the run via other
detection techniques. Note that the actual fraction
size may be less if a newly detected peak causes a
tube change or if you click the N
· Fraction volume may also be less than the max
volume if the system is configured with an ELSD or
MS.
UV or UV-Vis – Selecting a "λ" button allows you to specify the
wavelength for detection.
• Up to two different wavelengths can be monitored or
used to trigger fraction collection.
When enabled, clicking the D
O
window on which you can make additional settings.
PTIONS
Detection Options (λ) – A window with options to cus-
tomize the detection methods and parameters.
• Signal Gain – Modifies the gain to increase the detector
signal. This is a mathematical multiplication of the
signal to increase the size of peaks on the display..
• Slope Based – Allows peaks to be cut on the basis of the
slope of the signal.
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
window, or a
ONFIGURATION
T
button.
EXT
UBE
button opens a D
ETAILS
ETECTION
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