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CombiFlash NextGen Systems
Section 2 Preparation
2-24
2. There are 4 solvent selection lists labeled from first solvent
through fourth solvent, describing the order in which sol-
vents will be pumped. The solvent choices for each list are
those defined by the C
• If all solvents are of a similar polarity, it is recommended
that the solvent be primed in order of decreasing solvent
strength, with the weakest solvent primed last.
• If the 4 solvents are of mixed polarity (aqueous and
nonpolar), ensure that sequential solvents are
compatible, such as water, acetonitrile, ethyl acetate,
hexane.
• If you are performing an auto prime after redefining
some of the solvents, it may be beneficial to run 2
auto prime operations sequentially to get full flushing
of the lines.
3. Click the S
button ("
TART
Note
When priming the first time, inspect the solvent and waste con-
nections to the system. If any leaks are observed, click the
C
button to stop the Auto Prime. Correct the leak by
ANCEL
tightening the fitting an additional 1/4 turn, and then restart the
Auto Prime from step 1.
To terminate an auto prime step in process and advance to the
next Auto Prime step, you can click the T
Auto Prime window closes when finished.
After Auto Priming, the system is ready for operation. If you are
priming a new system for the first time, it may be beneficial to
flush all of the fluid lines with the desired solvent to remove any
isopropanol left from production testing.
Note
Use Auto Prime for quick solvent changes of similar polarity by
pumping a fixed amount of solvent through the waste fluid path
at 100 mL/min. For more advanced priming functions such as
varying the flow rates, fluid paths, etc., or to purge the system
with air before changing between normal and reversed phase
solvent systems, use the T
Manual Prime (NextGen models only) – The pumping
system in the base NextGen model requires the pumps to be
manually primed. To do this, use the syringe port on the front of
the instrument under the screen. From T
P
and follow the directions on the screen. Pull the syringe to
RIME
remove air from each of the solvent lines as directed by the on
screen instructions.
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
window.
ONFIGURATION
⏵ ") to start priming the system.
ERMINATE
> M
C
OOLS
ANUAL
ONTROL
select M
OOLS
button. The
option.
ANUAL
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