Table of Contents
Advertisement
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
The default settings include:
• Pre-configured equilibration settings based upon
column size and type.
• Flow rate and separation length settings suitable for the
column loaded.
• A setting to collect all fluid in the fraction collector rack
to ensure peaks without UV absorbance are collected.
• Pre-programmed peak detection settings suitable for
typical peak sizes based on the column size.
• Automatic advancement to the next tube when a peak is
detected and when a peak ends to maximize peak
concentration.
• Advancement to the next tube if a partially resolved
peak has a shoulder or valley UV absorbing peaks based
on slope detection or level threshold.
To start a separation from the P
1. Enter a sample name if desired. If no sample name is spec-
ified, PeakTrak will enter the date and time as the sample
name when you start the run.
2. Review the P
EAK
tings can be edited from the main menu. For example:
• If the gradient conditions are not correct, the duration
can be adjusted using the R
• The gradient slope can be adjusted by grabbing an
inflection point and dragging to the desired position.
• Inflection points can be added or deleted if needed.
Select the I
NSERT
where a point should be added, or D
touch the point to be removed.
• For greater detail, the screen can be zoomed using a
two-finger pinch zoom, or panned using two fingers.
• Other parameters may be edited such as peak detection
parameters or numerical gradient point input using the
M
E
ETHOD
DITOR
the top of the screen.
3. Click the S
button. The M
TART
window opens.
4. Select the S
AMPLE
type should support the sample preparation you chose.
Possible sample loading types are:
• Solid (requires the inject valve; optional on some
systems) – Select this option if you have prepared the
sample and placed it into a solid sample load cartridge
before starting the separation. (Column plan subscribers
must also specify the cartridge type and size).
· The system will proceed automatically until the end
of the programmed run length.
CombiFlash NextGen Systems
Section 3 Operation
T
M
EAK
RAK
AIN
T
M
window settings. Some set-
RAK
AIN
L
input field.
UN
ENGTH
P
button and touch the screen
OINT
ELETE
selection from the PeakTrack menu at
R
INIMUM
UN
L
type from the list. This loading
OADING
window:
P
and
OINT
R
EQUIREMENTS
3-3
Advertisement
Table of Contents
