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CombiFlash NextGen Systems
Section 3 Operation
3.7 Focused Gradients for
Flash Columns
3.7.1 Configuring a column
to use a scouting
gradient
Figure 3-17 Selecting a column from the Main Screen
3-24
conditions for your separation. Focused gradients offer a quick
way greatly improve resolution around the peaks of interest,
decrease overall solvent usage, and generate less waste solvent.
The Focus Gradient Generator is included in PeakTrak versions
5.1.7 and greater. (Press H
information.)
For a more detailed discussion of creating focused gradients for
flash columns and for useful tips, see Chromatography Appli-
cation Note TN65, Flash Method Development in a Flash. Also
see TN62, Reverse Phase Flash Method Development Using Ana-
lytical LC Systems to create focused gradients for analytical
HPLC columns.
The Focus Gradient Generator allows you to quickly create effi-
cient preparative gradient methods using flash columns
To use the Focus Gradient feature, you must first configure your
column to use a scouting gradient to determine where the target
compound elutes during the gradient. To that end, methods 1a or
1b instruct the system to load a pre-calibrated scouting run.
1. Do one of the following:
a. Just after installing a RediSep Gold column, the sys-
tem reads its RFID tag and a dialog appears. From the
dialog, select S
b. If the column is already installed, select a C
from the Main Screen and choose the S
2. Open the M
ETHOD
3. Press a peak detection D
O
as required.
TION
PTIONS
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
> A
P
ELP
BOUT
EAK
F
G
COUT FOR
OCUS
RADIENT
E
.
DITOR
button to set peak D
ETAILS
T
for version
RAK
.
size
OLUMN
method.
COUT
-
ETEC
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