Calibrating The System; Installing A Calibration Nanotile - Waters TRIZAIC UPLC System Manual

Calibrating the system

Calibrating the system involves adjusting the TRIZAIC UPLC source for
normal operation using a calibration nanoTile. The fittings do not require any
tools.
Caution:
• handle it with care. The components are fragile.
• do not subject it to pressures that exceed 34,474 kPa (345 bar,
5000 psi).
• do not apply electrospray potential to the emitter without flow.
• do not drop it, or immerse it in liquid.
• do not freeze or overheat it. Keep the nanoTile within the allowed
temperature ranges during operation and in storage.
• use the nanoTile sheath to protect the device when it is not in use.
• do not bend or pull the capillary connection tubing at the TRIZAIC
module coupling.
• avoid excess voltage, which can erode the emitter over time.
• do not touch the electrospray emitter, which can bend.

Installing a calibration nanoTile

Use the calibration nanoTile to optimize the spray position. (The device does
not contain chromatography particles.)
The topic "Preparing the TRIZAIC UPLC System" in the online
See also:
Help, for detailed instructions on performing the calibration procedure.
To install a calibration nanoTile:
1. Insert a calibration nanoTile into the docking port on the TRIZAIC
source.
See also:
information about installing and removing nanoTiles.
2. Rotate the lever to engage the fluid and electronic connections.
Result:
inserted into the TRIZAIC source's docking port. The light shows steady
green when the clamp is engaged, indicating it is ready for use.
To prevent damage to the nanoTile,
TRIZAIC UPLC nanoTile Care and Use Guide for detailed
The Status light blinks green when the nanoTile is correctly
Calibrating the system 2-13