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3.
Use the controls to adjust the position of the nanoTile until it matches
the cross-hairs.
On the camera view, the analytical nanoTile is usually within 0.25 mm
of the marked emitter position.
4.
Adjust the Z axis as necessary.
Preparing for sample analysis
To prepare for sample analysis:
1.
Create a nanoACQUITY Binary Solvent Manager instrument method
using the single-trapping fluid configuration.
•
In the analytical tab, select the A and B solvents, and enter the seal
wash period and the steps in the gradient table.
•
In the trapping tab, enter the flow rate, percentages of solvents A
and B, and sample loading time.
Limitation:
2.
Create a sample manager instrument method.
•
Select Partial Loop for the sample loop option.
•
Select water for weak wash and acetonitrile for strong wash.
•
Increase the weak wash to 1000 µL to improve retention of
hydrophilic peptides.
•
Enter 35 °C for the nanoTile temperature.
3.
In the MassLynx application, create a sample list, and then acquire
data.
Result:
Tips:
•
During the trapping phase, the gas is audible.
•
Click nanoTile in the console tree for current nanoTile information.
•
The EEPROM in the nanoTile stores information such as usage and
nanoTile history of sample sets.
2-20
Preparing the System
The high pressure limit is 34,500 kPa (345 bar, 5000 psi).
The TRIZAIC UPLC System is ready to inject samples.
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