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Waters ACQUITY UPLC Peptide HSS T3 100A Care And Use Manual
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Waters ACQUITY UPLC Peptide HSS T3 100A Care And Use Manual

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[ CARE AND USE MANUAL ]
ACQUITY UPLC Peptide HSS T3, 100Å Columns
CONTENTS
I.
INTRODUCTION
II.
II.
III.
IV.
V.
VI.
VII.
I. INTRODUCTION
Thank you for choosing a Waters® ACQUITY UPLC® Peptide
HSS T3, 100Å Column that contains our High Strength Silica
particle technology to deliver increased peptide retentivity
and different selectivity compared to results generated on
the Peptide CSH™ C
, 130Å or Peptide BEH C
18
300Å Column offerings. The manufacture of our Peptide
HSS T3 particles begins with ultrapure reagents to control
the chemical composition and purity of the final product.
Peptide HSS T3 columns are manufactured in a cGMP, ISO
9001:2000 certified plant with each step being conducted
within narrow tolerances. Each batch of ACQUITY UPLC
HSS T3, 100Å material is chromatographically tested with
acidic, basic, and neutral analytes, as well part of Waters'
standard batch qualification procedure. In addition, each
batch of HSS T3, 100Å material is QC tested with a gradient
separation of a tryptic digest of cytochrome c and must pass
stringent performance criteria before acceptance for use in
an ACQUITY UPLC Peptide HSS T3, 100Å Column. A final
QC test for packed bed efficiency is also performed on each
column with this as well as the batch test results provided on
the column's attached eCord™ Intelligent Chip.
ACQUITY UPLC Peptide HSS T3, 100Å Columns were designed
and tested specifically for use on ACQUITY UPLC Systems and
will deliver maximum chromatographic performance when
used on the holistically-designed ACQUITY UPLC System.
, 130Å and
18
1

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Summary of Contents for Waters ACQUITY UPLC Peptide HSS T3 100A

  • Page 1: Table Of Contents

    Each batch of ACQUITY UPLC COLUMN USE HSS T3, 100Å material is chromatographically tested with a. Sample Preparation acidic, basic, and neutral analytes, as well part of Waters’ b. pH Range standard batch qualification procedure. In addition, each c. Solvents batch of HSS T3, 100Å...

  • Page 2: Getting Started

    1. Perform an efficiency test on the column before using it. phases and connect the inlet end of the column to the Waters recommends using a suitable solute mixture, as injector outlet. found in the “Performance Test Chromatogram”, to analyze 2.

  • Page 3: Vanguard Pre-Columns

    [ CARE AND USE MANUAL ] f. VanGuard Pre-Columns 5. While pushing the VanGuard Pre-column into the column inlet, turn assembled column and pre-column 180˚ so that VanGuard™ Pre-columns are 2.1 mm I.D. x 5 mm length guard the pre-column is now on top. column devices designed specifically for use in the ACQUITY UPLC®...

  • Page 4: Ph Range

    Increasing column temperature increases cleaning efficiency. If the column performance is poor after regenerating and d. Pressure cleaning, call your local Waters office for additional support. ACQUITY UPLC Peptide HSS T3 Columns can tolerate operating pressures up to 18,000 psi (1241 bar or 124 MPa).

  • Page 5: Ecord Intelligent Chip Technology

    QC test conditions and results on the column run by the manufacturer. The information includes mobile phases, running Figure 1. Waters eCord Intelligent Chip. conditions, and analytes used to test the columns. In addition, the QC results and acceptance is placed onto the column.

  • Page 6: Additional Information

    [ CARE AND USE MANUAL ] V. ADDITIONAL INFORMATION Don’t: assume a “bad” column is the culprit when high backpressure or split peaks are observed: a. Tips for Maximizing ACQUITY UPLC Peptide HSS T3 Investigate cause of column failure ■ ■ Column Lifetimes Backpressure To maximize ACQUITY UPLC Peptide HSS T3 Column...

  • Page 7: Representative Test Chromatogram

    34 Maple Street Milford, MA 01757 U.S.A. Waters, The Science of What’s Possible, and ACQUITY UPLC are registered trademarks of Waters Corporation. CSH, eCord, T: 1 508 478 2000 and VanGuard are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.