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Focusing on the sprayer tip:
Optimal position of sprayer tip
To prevent damage to the electrospray emitter,
Caution:
• do not apply capillary voltage to the emitter unless solvent is flowing
to the nanoTile. Doing otherwise raises the optimal electrospray
potential and limits the lifetime of the emitter.
• avoid arcing to the baffle, which can cause excess oxidation and
reduce the emitter's lifetime.
Recommendations:
•
A suggested starting range for the capillary voltage is
approximately 3.0 kV to 3.4 kV. Both position and flow rate affect
the optimal voltage. The higher the flow rate, the greater the
optimal voltage.
•
Use a calibration solution with the same solvent composition as the
gradient. For example, if the majority of analytes elute at
25% organic solvent, use a calibration solution of 25% organic
solvent.
•
During calibration, use the same flow rate as the intended flow rate.
If the gradient flow rate changes, adjust the spray position and
capillary voltage.
5.
Switch the baffle to the lock-spray position, to make sure the emitter
does not arc to the baffle. If you detect arcing, retract the emitter
Sample cone
Baffle
Calibrating the system
2-17
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