Run Sample - Agilent Technologies NovoCyte Quanteon Operator's Manual

Running Samples

Run Sample

There are two samples for the example in this guide. Double-click each sample to
make it to be the Active Sample (red arrow shown on the left of the sample).
Define the collection parameters and set the acquisition conditions for the two
samples as shown in Figure 46.
Figure 46. Instrument Settings Panel
After setting the acquisition parameters and acquisition conditions, double-click
the T-Lym sample and set it as the Active Sample in the Experiment Manager. A
red arrow will appear to the left of the sample, indicating it is the Active Sample. At
the same time, the sample name in Cytometer Control > Active Sample
Information panel becomes T-Lym. To begin collecting this sample, place the CD3
FITC/CD8 PE/CD45 PerCP/CD4 APC sample tube into the sample holder on the
NovoCyte Quanteon flow cytometer. Then click the Run button in the Cytometer
Control > Experiment Control panel to start sample acquisition (Figure 47). At this
time, the sample injection probe will move downward to aspirate the sample. After
a short period of time for stabilizing the fluidic system, sample acquisition and
data collection will start. During the data acquisition, the Restart button will appear
in the Experiment Control panel. Clicking this button will delete all collected
events, reset the collection time and volume in the Active Sample Information
panel, and restart the data collection.
80 NovoCyte Quanteon Flow Cytometer Operator's Guide