Agilent Technologies NovoCyte Quanteon Operator's Manual page 112

Flow cytometer
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System Maintenance
4
Power on the instrument and start NovoExpress software. After the
instrument is Ready, click Instrument > Fluidics Maintenance > Cleaning to
finish.
SIP cleaning apparatus directly contacts the biological samples which may
WARNING
be biohazardous. Wear gloves when handling the SIP cleaning apparatus.
When cleaning the SIP cleaning apparatus, please be careful to prevent any
CAUTION
damages to the apparatus and sample injection probe.
Unclog the Flow Cell
When the instrument is in Ready status, click Instrument > Fluidics Maintenance >
Unclog from NovoExpress software, and the Unclog procedure will automatically
be executed. The progress of the procedure will be shown in the NovoExpress
status bar.
Figure 87. Unclog Function in NovoExpress
Sterilize Instrument Reagent Containers
It is very important to keep the instrument reagent free of contamination by
sterilizing the instrument reagent containers. This procedure is highly
recommended once a month or whenever there is a suspected bacteria growth or
possible contamination (e.g. by mishandling the containers). The reagent
containers need to be properly removed and sterilized using the following method
respectively.
Please refer to
NOTE
containers.
The procedures described below may vary if NovoCyte Fluidics Cart is used.
NOTE
Please refer to
1
Ensure the container is properly removed from the fluidics station. Wipe the
outer surface of the container using clean soft cloth soaked with 70% ethanol.
2
Empty the reagent container. Fill the NovoFlow container with at least 300 mL
of NovoClean solution (1X) and cap the bottle. Fill the NovoClean and
112
Instrument Start-Up
in this guide for instruction on removing the
NovoCyte Fluidics Cart Operator's Guide
NovoCyte Quanteon Flow Cytometer Operator's Guide
for more details.

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