Replacing Fluidic System Consumables - Agilent Technologies NovoCyte Quanteon Operator's Manual

Flow cytometer
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System Maintenance
NovoRinse container with at least 100 mL of NovoClean solution (1X) and cap
the bottle. Shake the container vigorously to let the NovoClean solution cover
the entire inner surface. Repeat for 3 times and then soak for 30 min.
3
Empty the reagent container again. Fill about 500 mL Milli-Q or 0.2 µ m filtered
DI water (or NovoFlow solution) to NovoFlow container and cap the bottle. Fill
about 100 mL Milli-Q or 0.2 µ m filtered DI water (or NovoFlow solution) to
NovoClean and NovoRinse container and cap the bottle. Shake reagent
container for at least 2 minutes.
4
Repeat Step 3 three times.
5
Fill the reagent container all the way to the neck or close to the brim with Milli-
Q or 0.2 µ m filtered DI water or NovoFlow sheath fluid. Soak for 20 min and
empty the reagent container.
6
Wipe the outer surface of the container with a dry and clean soft cloth.
7
Place the container back onto the fluidics station.

Replacing Fluidic System Consumables

NovoExpress software monitors the accumulated running time of the fluidic
system consumables to ensure the consumables are changed in a timely manner
for optimal flow cytometry results. When the accumulated running time is reached,
the NovoExpress software will prompt a message to remind the user to replace
the consumables (Figure 88). A similar message will be prompted when the last
replacement is 60 days ago as shown in Figure 89.
Click Replace to continue. Clicking the Close button will cancel the process and
allow continued use of the system. However, the consumables should be replaced
promptly.
Figure 88. Software Message Indicating Fluidic System Consumables Reached Working Capacity
NovoCyte Quanteon Flow Cytometer Operator's Guide
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