Agilent Technologies NovoCyte Quanteon Operator's Manual page 99

Running Samples
Figure 72. CD45 PerCP-H/SSC-H Density Plot
The NovoCyte Quanteon flow cytometer has a very large dynamic range (0 to
2²⁴=16,777,216), so the data will often only occupy a small area of the plot when
using the full-scale axes. Zoom-in to enlarge the data area of interest. To zoom in,
click the zoom-in icon
selected plot and select Zoom In from the drop-down list. Move the mouse across
a rectangular area to zoom in the population of interest. Upon release of the
mouse button, the range of the plot is automatically enlarged according to the
zoom-in operation. You can also Undo the Zoom In operation by clicking the undo
icon
the data of interest is properly shown on the plot as shown in Figure 73.
Figure 73. Zooming in the Density Plot
Create a Gate for Lymphocytes
•
Click the elliptical gate icon
population (large PerCP signal and small SSC signal) on the CD45 PerCP-H/SSC-H
density plot. The default name of the gate is E1 (E indicates an elliptical gate). Click
the E1 gate label and use the keyboard to change the name of the gate to
Lymphocyte.
Create a CD3 FITC-H/CD8 PE-H and a CD3 FITC-H/CD4 APC-H Dot Plot
•
NovoCyte Quanteon Flow Cytometer Operator's Guide
from the data analysis toolbar, or right-click on the
or click the zoom-out icon from the toolbar. Repeat the above steps until
, draw an elliptical gate to include the lymphocyte
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