Agilent Technologies NovoCyte Quanteon Operator's Manual page 135

Troubleshooting
not connected even
though the
instrument is
powered up
Unstable Signal or
gap in the data plot
Sample tube does
not fit the sample
tube holder
Sample Injection
Probe is not
properly aligned
with the wells or
tubes
Leakage from the
SIP
Leakage from
Sampling Pump
Leakage from other
components of the
instrument
NovoCyte Quanteon Flow Cytometer Operator's Guide
communication between the
instrument and workstation
Improper connection between
the instrument and
workstation
More than one NovoExpress
instances are running
Air bubbles exist in the flow
cell
Wrong type of sample tube is
used
Plate or tube rack is not
loaded correctly.
NovoSampler (or
NovoSampler Pro or
NovoSampler Q) is not
calibrated.
Waste tubing is deformed
Waste filter is clogged
Incorrect tubing connection to
the sampling pump
Incorrect pump or valve or
tubing connection or bad
sheath in-line filter
Reconnect the USB cable between the
instrument and the workstation.
Only run one instance of NovoExpress software.
Execute the Debubble function from
NovoExpress Software.
Use 12 × 75 mm tube or 1.5 mL tube 2.0 mL
tube.
Load the plate or tube rack with the correct
orientation. Make sure the plate or tube rack is
fully seated onto the sample tray properly.
Re-calibrate the NovoSampler (or NovoSampler
Pro or NovoSampler Q). Refer to associated
operator's guide for more details.
•
Reconnect the waste tubing to ensure it is
properly connected.
•
Move the instrument forward as needed to
ensure sufficient space between the
instrument and wall. Refer to associated
instrument operator's guide for more
information on properly installing
instrument.
Replace the waste filter following the
procedures described in associated instrument
operator's guide.
Reconnect the tubing to the sampling pump.
Turn off the instrument and contact Agilent
technical support for assistance.
135