Introduction; Overview; Basic Principle; Optics System - Agilent Technologies NovoCyte Quanteon Operator's Manual

Introduction

2 Introduction
This chapter provides overview of NovoCyte Quanteon flow cytometer, including
basic instrument operation principle, system requirements, and system
components.

Overview

Agilent flow cytometer can be used for scientific research, including but not limited
to detection and analysis of cell surface antigens, intracellular activity analysis,
detection and analysis of apoptosis, DNA content detection, cell cycle analysis of
the nucleus, analysis of cell proliferation, and GFP reporter gene detection, etc.
Agilent flow cytometer can detect a particle with the size of 0.1 μm - 50 μm.
Besides human cells, it can also be used to analyze:
Animal Cells
•
Plant Cells
•
Marine plankton
•
Bacteria
•
Fluorescent microspheres
•

Basic Principle

Agilent flow cytometer contains the optics system, the fluidics system, and the
electronics system. This section describes the basic principle of these subsystems
and their components.

Optics System

The optics system consists of lasers, optical lenses, beam shaping optics, light
collection optics, optical filters, and photodetectors. Lasers illuminate the cells or
NovoCyte Quanteon Flow Cytometer Operator's Guide 29