Set Sample Acquisition Conditions; Stop Condition - Agilent Technologies NovoCyte Quanteon Operator's Manual

Running Samples
The set of parameters available in the Parameters panel is dependent on the model
NOTE
of NovoCyte Quanteon flow cytometer. The software will automatically identify the
instrument configuration and detect the available channels to be shown in
Cytometer Setting > Parameters panel.
De-selection of parameters that are not used in the experiment will help to reduce
NOTE
the size of the experiment file, and thus improve the data analysis speed.
The Gain column shows photodetector gains of all fluorescent channels. NovoCyte
NOTE
Quanteon uses fixed photodetector gain by default. Adjustable photodetector gain
is available as advanced function. Please refer to the
for details about photodetector gain adjustment.

Set Sample Acquisition Conditions

Stop Condition

The criteria to stop sample acquisition can be set in Cytometer Setting > Stop
Condition panel (Figure 43), based on either the number of events to be collected
(all events or within a specified gate), a time limit, or a sample volume limit.
Multiple stop conditions can be selected as well. When any one of these
conditions is met, the instrument will stop collecting samples. The number of
events to be collected may be modified during the sample collection process.
However, the time or volume stop conditions cannot be modified.
Figure 43. Set Stop Condition
76 NovoCyte Quanteon Flow Cytometer Operator's Guide
NovoExpress Software Guide