Agilent Technologies NovoCyte Quanteon Operator's Manual page 134

Troubleshooting
High CV
Excessive debris in
display
High event rate
NovoExpress
displays Instrument
134
NovoFlow container is empty
NovoFlow container is not
correctly connected to the
instrument and the NovoFlow
solution does not flow through
the tubing
Sample Injection Probe is
clogged
Flow cell is clogged
Sample tubing is leaking
Air bubbles exist in the
Sampling Pump
Air bubbles exist in the flow
cell
Flow cell is clogged
Improper sample preparation
The sample flow rate is set
too high
Sheath in-line filter is dirty
The threshold is set too low
Fluidics system tubing is dirty
The threshold is set too low
Sample concentration is too
high
Sample flow rate is too high
Workstation is in hibernation
causing the interruption of
NovoCyte Quanteon Flow Cytometer Operator's Guide
Refill the NovoFlow container following the
procedures described in associated instrument
operator's guide.
Reconnect the NovoFlow quick coupling
connectors to ensure the NovoFlow container is
correctly connected to the instrument.
Execute Prime function from the NovoExpress
Software.
Run NovoClean as sample to remove the things
clogged in SIP.
Execute the Unclog function from the
NovoExpress Software.
Clean the leaked fluid with wipes and properly
reconnect the sample tubing.
Execute Debubble function from NovoExpress
Software.
Execute the Debubble function from
NovoExpress Software.
Execute the Unclog function from NovoExpress
software
Re-prepare the sample properly.
Lower the sample flow rate setting in the
NovoExpress software.
Replace the sheath in-line filter following the
procedures described in associated instrument
operator's guide.
Increase the threshold setting and re-collect the
sample.
•
Execute Rinse function from NovoExpress
Software.
•
Execute Cleaning function from
NovoExpress Software.
•
Execute Decontamination function from
NovoExpress Software.
Increase the threshold setting and re-collect the
sample.
Re-prepare the sample to appropriate
concentration or dilute the sample.
Reduce the sample flow rate.
•
Restart NovoExpress Software.
•
Restart workstation.