Agilent Technologies NovoCyte Quanteon Operator's Manual page 75

Running Samples
B525, Y586, B667, and R667 to be CD3 FITC, CD8 PE, CD45 PerCP, and CD4
APC, respectively.
Figure 42. Define Sample Acquisition Parameters
Parameters that are not selected in the Parameters setting panel will not be
NOTE
collected in the data, and therefore will not be available for data analysis. However,
selecting too many parameters other than those really needed in the experiment
will unnecessarily increase the size of the experiment file, causing decreased data
processing speed. Before doing an experiment, the parameters should be set
appropriately, in order to avoid incomplete data collection or slow software data
processing speed.
After the system has started collecting samples, only the Alias of the parameters
NOTE
can be edited. Selection or de-selection of parameters is not allowed.
NovoCyte Quanteon Flow Cytometer Operator's Guide 75