Agilent Technologies NovoCyte Quanteon Operator's Manual page 91

Running Samples
If a channel parameter does not need to be included for the compensation,
click the blank area in the pull-down menu. The check box in front of the
corresponding channel parameter will be automatically unselected.
The software will automatically associate the imported FCS file with the selected
NOTE
channel parameter based on the keyword of the file name (e.g. B525). Users need
to verify and select the correct FCS file for each fluorescence detection channel.
Figure 60. Set the Autocompensation Parameters
5 Click OK and NovoExpress will automatically calculate the compensation
matrix based on the samples imported. Once generated, the compensation
matrix can be applied to other experiment samples.
NovoCyte Quanteon Flow Cytometer Operator's Guide 91