Agilent Technologies NovoCyte Quanteon Operator's Manual page 100

Running Samples
Click the dot plot icon
parameter to be CD3 FITC-H and the Y-axis to be CD8 PE-H for the first dot plot.
Change the X-axis parameter to be CD3 FITC-H and Y-axis to be CD4 APC-H for
the second dot plot. Right-click the name of both plots and select Lymphocyte
from the list to apply the Lymphocyte gate (created in CD45 PerCP-H/SSC-H
density plot) to both plots. Observe the change after application of the gate.
Compensate the Sample
•
Use the method described in
apply the compensation matrix created by either automatic compensation or
manual compensation to the sample. Figure 68 and Figure 69 show the data for
the T-Lym sample before and after compensation.
Statistics
•
In the CD3 FITC-H/CD8 PE-H and CD3 FITC-H/CD4 APC-H dot plots, create a
quadrant gate to isolate the positive and negative cell populations. Click the small
triangle at the bottom-right of the plot to expand the statistical results of the data,
as shown in Figure 74. Right-click any place on the statistical table to select
additional statistical parameters to be calculated for the data, as shown in Figure
75.
Figure 74. Statistical Data
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twice to create two new dot plots. Change the X-axis
Perform Fluorescence Compensation
NovoCyte Quanteon Flow Cytometer Operator's Guide
in this guide to