Add Instrument Reagents - Agilent Technologies NovoCyte Quanteon Operator's Manual

Instrument Operation
The NovoExpress Software initially includes an administrator account. The default
NOTE
password for this account is administrator. The administrator login allows for
creation of additional users and control of password settings. Please refer to the
NovoExpress Software Guide

Add Instrument Reagents

If the reagent level is below 1/3 capacity on any reagent container, follow the
instructions below to add more reagent to the container.
1 Make sure the instrument is in Ready status or the power is turned off.
2 Disconnect the tubing from the corresponding container by gently pressing the
metal clip of the quick coupler on the cap.
3 Move the container from the NovoCyte Fluidics Station II to a sink. Unscrew
the container cap and add more reagents.
4 Screw the container cap back onto the container.
5 Reconnect the quick coupler on the cap and gently place the container onto
the NovoCyte Fluidics Station II. Be careful to prevent any twisting or kinking of
the tubing.
6 Ensure the instrument is in Ready status, click the Instrument > Fluidics
Maintenance > Priming on the NovoExpress software to prime the fluidics
system before running any samples.
It's necessary to run Priming after adding instrument reagent. Otherwise the air
NOTE
introduced into tubings during adding reagent will influence the instrument
performance.
Please make sure the instrument is in Ready status or powered off before
CAUTION
adding any instrument reagents.
Be sure to correctly connect the corresponding reagent container to the
CAUTION
fluidics port on the back of the instrument. Incorrect connection will cause
the instrument malfunction. The tubing and containers are color coded to
prevent such errors.
NovoCyte Fluidics Station II contains pressure sensors that may be
CAUTION
damaged by spilled liquid. To prevent the risk of sensor damage, do not
add reagent while the containers are on the NovoCyte Fluidics Station II.
NovoCyte Quanteon Flow Cytometer Operator's Guide
for details.
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