Agilent Technologies Varian CP-8400 Operator's Manual
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Agilent Technologies Varian CP-8400 Operator's Manual

Agilent Technologies Varian CP-8400 Operator's Manual

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NOTICE: Varian, Inc. was acquired by Agilent
Technologies in May 2010. This document is provided
as a courtesy but is no longer kept current and thus
will contain historical references to Varian. For more
information, go to www.agilent.com/chem.
CP-8400 AutoSampler and
Varian, Inc. 2003
CP-8410 AutoInjector
Operator's Manual
Printed in the Netherlands
Varian, Inc.
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/USA
03-914852-00:Rev. 4.1

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  • Page 1 NOTICE: Varian, Inc. was acquired by Agilent Technologies in May 2010. This document is provided as a courtesy but is no longer kept current and thus Varian, Inc. will contain historical references to Varian. For more 2700 Mitchell Drive information, go to www.agilent.com/chem. Walnut Creek, CA 94598-1675/USA CP-8400 AutoSampler and CP-8410 AutoInjector...

  • Page 2: Table Of Contents

    Table of Contents Introduction..........................1 Installing the CP-8400/8410 ....................3 Configuring your CP-8400/8410 to use SPME ..............9 Setup and Calibration of the CP-8400/8410 ................ 11 Vial 0 / Vial 1 Calibration ......................17 Vial Calibration tool........................19 Injector Calibration........................21 Operation ..........................

  • Page 4: Introduction

    Introduction We strongly recommend that you read the entire manual before you start using your CP-8400 AutoSampler or CP-8410 AutoInjector. The CP-3800 will sense whether you have a CP-8400 or CP-8410 installed automatically and will sense if the Auto Sampler is set to perform liquid sampling with a syringe or SPME sampling.
  • Page 5 Your AutoSampler or AutoInjector will execute the desired injection. To set up an automated series of injections, simply complete the following steps: 1. Press the Automation Select/Edit button on the CP-3800, choose either Single Method automation to run all of your samples with a single method, or choose Method Sequence Automation to run specific methods with specific vials.

  • Page 6: Installing The Cp-8400/8410

    Installing the CP-8400/8410 To use the CP-8400 with the CP-3800 Gas Chromatograph, your CP-3800 must have Version 3.2 or later software installed. The CP-8410 requires version 3.3 or later. To use SPME mode sample introduction with either the CP-8400 or CP-8410 you must have Version 3.3.5 or later software installed.
  • Page 7 Injector Locator Plate CP-8400 in Front Position CP-8400/8410 CP-3800 Top Cover To install the CP-8400/8410, follow the steps below: WARNING: SHOCK HAZARD Dangerous voltage exposed. Turn the CP-3800 GC power off. Failure to do so may result in accidental contact with dangerous voltage. 1.
  • Page 8 Installing the CP-8400/8410 CP-8400/8410 Power/Communication Connection to CP-3800 Aux 1 Back of CP-8400/8410 CP-8400/841- Power Switch Cables routed through hole in cover Cable Routing for CP-3800 CP-8400 AutoSampler & CP-8410 AutoInjector...
  • Page 9 4. If the Injector locator plate is installed in the top cover, remove it by removing the two screws located on either end of the locator plate. Install the top cover so that the pins on the top cover fit into the corresponding slots on the CP-3800 chassis.
  • Page 10 Installing the CP-8400/8410 Screw Holes: Used in mounting both front and rear AutoSampler positions. Screw hole used in Screw hole used in mounting rear position mounting front position only. only. Carrousel Removed to Show Mounting Screw Locations 8. Plug the D-shell connector of the power/communications cable into the CP-8400/8410.
  • Page 11 Plunger Retaining Nut (unscrew to remove syringe and tighten when installing) Syringe Retaining Clip (lift up to remove syringe) Syringe Installation 10. If the currently installed CP-3800 ROM is version 3.1 or lower, replace with a version 3.3.5 or higher ROM board. To use the CP-8400 with the CP-3800 Gas Chromatograph, your CP-3800 must have Version 3.2 or later software installed.

  • Page 12: Configuring Your Cp-8400/8410 To Use Spme

    Configuring your CP-8400/8410 to use SPME The Auto Sampler will only come up in one of two configurations. The first and default configuration is the liquid sampling configuration. In this configuration your Auto Sampler will use a syringe to withdraw liquid for subsequent injection into the chromatograph.
  • Page 13 7. Close the cover on the tower and power up the GC. The GC will automatically sense the configuration of the switch and behave accordingly. switch Note that sled has been pushed down compressing the foot and guide rail in order to reveal switch.

  • Page 14: Setup And Calibration Of The Cp-8400/8410

    Setup and Calibration of the CP-8400/8410 WARNING: BURN HAZARD The injector nut and locator plate may be very hot during instrument operation and should not be touched with unprotected hands. WARNING Syringe moves automatically during operation of instrument. Keep your hands and fingers out of the injection area.
  • Page 15 Calibrate both the sampling and injector positions of the CP-8400/8410 the first time you install it. Calibrate the injector positions any time you move the CP-8400/8410 from one position to the other (for instance from the front mounting position to the rear position). If calibration fails on either the vial 0 or the injector, you must recalibrate both the injector and vial 0.
  • Page 16 Setup and Calibration of the CP-8400/8410 EDIT ACTIVE CTRL METHOD TIME TIME METHOD EDIT INSTRUMENT SETUP MENU [ 1 ] Edit Time and Date [ 2 ] Edit Heated Zones [ 3 ] Edit EFC [ 4 ] Edit Column Parameters [ 5 ] Edit Sample Delivery Setup [ 6 ] Edit Miscellanous Setup Parameters Save and Exit...
  • Page 17 EDIT ACTIVE CTRL TIME METHOD TIME METHOD EDIT CP-8400 SETUP Injector Ports To Use With CP-8400: Both 10 µl Syringe Size: Save and Exit Calibrate Automation Configuration Select the Calibrate softkey to begin the CP-8400 (or CP-8410) calibration process. Injector Ports to use with CP-8400/8410 – Available entries: Position 1, Position 2, Both.
  • Page 18 Setup and Calibration of the CP-8400/8410 To begin calibrating the CP-8400/8410, press the Calibrate softkey at the bottom right corner of the Edit CP-8400 (or CP-8410) Setup screen. If you press Save and Exit without calibrating, the existing calibration parameters will be retained. ACTIVE EDIT CTRL...
  • Page 19 of the first vial in the carrousel (vial 0) or the middle of the first 2 mL vial of the AutoInjector sample tray. The CP-8400 may use a number of different sample vial form factors; however, you should use all of the same vials for each session. Mixing different vials is strongly discouraged.

  • Page 20: Vial 0 / Vial 1 Calibration

    Setup and Calibration of the CP-8400/8410 Vial 0 / Vial 1 Calibration ACTIVE EDIT CTRL METHOD TIME METHOD TIME CP-8400 VIAL 0 CALIB Component Direction 1 Direction 2 Tower Carrousel Syringe Down Start Calib. Move Finish Calib. ACTIVE EDIT CTRL METHOD TIME METHOD...
  • Page 21 To start the Vial 0 (or Vial 1) calibration, make certain a vial with the same form factor and cap as the ones you will be using for your analyses is placed in the 0 position of the carrousel (or the Vial 1 position of the CP-8410).

  • Page 22: Vial Calibration Tool

    Setup and Calibration of the CP-8400/8410 Vial Calibration tool In order to accurately calibrate vial positions, a calibration tool (CP740889) is used. Vial calibration tool The calibration tool simulates a standard vial. The tool is used for both the CP-8400 and CP-8410 AutoSamplers. The calibration tool can be placed (depending of the tray-material) in two different ways in the tray.
  • Page 23 When you have completed the Vial 0 (or Vial 1) calibration, press the Finish Calib. softkey. When “Finish Calib.” is done, the softkey will change from “Reverse Video” to Normal, indicating the calibration process for this page is finished. You may either Save and Exit to store the new Vial 0 calibration values by pressing the Page Up key two times and then the Save and Exit keys, or you may continue to the Injector Position calibrations by pressing the Next Page key on the CP-3800...

  • Page 24: Injector Calibration

    Setup and Calibration of the CP-8400/8410 Injector Calibration ACTIVE EDIT CTRL METHOD TIME METHOD TIME CP-8400 INJ 1 CALIB Component Direction 1 Direction 2 Tower Syringe Down Start Calib. Move Finish Calib. To calibrate the Injector 1 and Injector 2 Positions, perform the following steps: 1.
  • Page 25 4. Press the Finish Calib. softkey when the Injector 1 calibration is complete. You may either press the Page Down key on the CP-3800 keypad to continue to the Injector 2 calibration (follow the instructions above to calibrate the Injector 2 position) or Page Up three times and press the Save and Exit softkey to save the values and restart your CP-3800.

  • Page 26: Operation

    Operation Parameters and Quick Reference The layout of the screens and the placement of the parameters followed the philosophy of keeping those parameters that an operator may wish to change for a series of samples (such as the injection volume, vial range, GC method) within the Automation pages for quick access and change.
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  • Page 28 Operation CP-8400 AutoSampler & CP-8410 AutoInjector...
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  • Page 30 Operation CP-8400 AutoSampler & CP-8410 AutoInjector...

  • Page 31: Automation Page Parameters

    Automation Page Parameters The CP-8400/8410 has a Single Method automation mode and a Method Sequence mode. The Single Method Automation consists of one page if the Setup parameter Number of Injection Ports is set to either Position 1 or Position 2, or two pages if the Number of Injection Ports in Setup is set to Both.
  • Page 32 Operation Method (Method 1 to Method 8) – This parameter selects which of the local methods will be used for single automation. Default selection will be Method 1. Initial Sample (0 to 99 for the CP-8400, 1 to 21 for the CP-8410) – The Initial Sample represents the vial position of the first vial to be used with the specified method.
  • Page 33 injection volume used. The volume is settable in 0.1 µL increments for the 5 and 10 µL syringes and 1 mL increments for the 100 µL syringe. The default value is 1 µL irrespective of the configured syringe volume. This volume will be used with the first injection made. This may be into either injector 1 or injector 2 depending on the First Injector Used parameter described below.
  • Page 34 Operation force the CP-8400/8410 to inject into both injectors for each run. The default value is 1. First Injector Used (1, 2) – This parameter is only used if the Injector Position is set to Both. This parameter defines which of the injectors will be used to inject into first and thus start the run.

  • Page 35: Method Sequence Table Parameters

    Method Sequence Table Parameters Page 1 (table) * denotes CP-8410 Method Vial Range Inj/Samp Inj Vol 2 Inj Vol 1 Inj Pos entries 1, 2, ..., 8 0 to 99 1 to 99 0.1 to 5, 10 0.1 to 5, 10 1, 2, or (1 to 21)* or 1 to 100...
  • Page 36 Operation Injection volume 1 & 2 specifies the volume of the sample that will be injected for the first and second injection respectively. (The First Injector Used parameter defines which of the two injector positions is injected into first.) This volume will be either 0.1 to 5 µL in 0.1 µL increments or, 0.1 to 10 µL in 0.1 µL increments, or 1 to 100 µL in 1 µL increments, depending on the volume of the installed syringe.

  • Page 37: Priority Sample

    This will prevent having the contents of the second vial being injected into both position 1 and position 2. If the SampleList is already at the end of the selected range, the second injection is not performed and the message ‘ No more vials in selected range’ displayed in the message line.

  • Page 38: Method Parameters

    Operation Page 2 (only displayed if Both is set in Setup) Injector Position Pos. 1, Pos. 2, Both default = Pos. 1 First Injector Used Pos. 1, Pos. 2 default = Pos. 1 Use Injection Delay? Yes or No default =No Delay Between Injections (min) 0.5-10.0 default = 1...

  • Page 39: Standard Split/Splitless And Standard On-Column Sampling Modes

    4. Solvent plug (if defined) 5. Upper Internal Standard Air Gap (if defined) 6. Internal Standard (if defined) 7. Upper Sample Air Gap (if defined) 8. Sample 9. Air Plug After Sample (if defined) There are six liquid injection modes (Standard On-Column, Standard Split/Splitless, Neat, Volatile, Viscous, User Defined) and SPME–...
  • Page 40 Operation The Standard On-Column and Standard Split/Splitless entries and available screens are summarized below: CP-8400 AutoSampler & CP-8410 AutoInjector...

  • Page 41: Neat Sampling Mode

    Neat Sampling Mode Internal Standard is not permitted with Neat Sampling mode. Neat mode parameters are set for samples that are normally liquids that will be injected without dilution. The Neat sampling mode performs the following sequence of actions: 1. Any pre-injection Clean actions will be executed. 2.
  • Page 42 Operation The Neat mode screens and parameters are displayed below: CP-8400 AutoSampler & CP-8410 AutoInjector...

  • Page 43: Volatile Sampling Mode

    Volatile Sampling Mode This mode is designed for samples dissolved in highly volatile solvents such as pentane. • The number of pre-injection solvent clean flushes specified in the method will be performed by drawing a full syringe of solvent from the specified clean vial and expelling it into the waste cup.
  • Page 44 Operation The Volatile mode screens and parameters are displayed below: CP-8400 AutoSampler & CP-8410 AutoInjector...

  • Page 45: Viscous Sampling Mode

    Viscous Sampling Mode Viscous mode is designed for samples that either are viscous or are dissolved in a viscous solvent such as cyclohexanol. Viscous mode actions are summarized below: • The number of pre-injection solvent clean flushes specified in the method will be performed by drawing a full syringe of solvent from the specified clean vial and expelling it into the waste cup.
  • Page 46 Operation The Viscous mode screens and parameters are summarized below: CP-8400 AutoSampler & CP-8410 AutoInjector...

  • Page 47: Spme Sampling Mode

    SPME Sampling Mode SPME mode is designed for using the special Solid Phase Micro- Extraction fibers and fiber holder. Note that you can not select the SPME Sampling mode from the CP- 3800 keypad. In order to configure the system to use SPME you must set a switch on the syringe sled to the correct position and install the special SPME fiber holder block.
  • Page 48 Operation Adsorb Time. Use the Sample Penetration Depth to sample from either headspace (the vapor above the sample) or into the liquid. The fiber holder sheath (the needle at the end of the fiber holder) will penetrate about 6 mm into the sample vial. The fiber will be extended varying distances based on the penetration depth setting.
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  • Page 50: User Defined Sampling Mode

    Operation User Defined Sampling Mode Allows access to all AutoSampler parameters. This mode of operation should be used with caution since some parameters, if not optimally set, can produce poor area precision and anomalous results. User Defined mode has three pages of parameters, and softkeys leading to additional parameters.
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  • Page 52 Operation Page 1 Parameters and Softkeys Sample Penetration Depth (0-100%) – This parameter defines how far the needle penetrates through the septum into the sample vial. The percentage refers to the distance from the bottom of the vial septum to the bottom of the vial interior.
  • Page 53 Internal Standard Selecting this softkey will display a page with the following parameters: Use Internal Standard? (Yes or No) – This parameter determines whether the system will use an Internal Standard as part of the sampling procedure. Selecting Yes will cause the CP-8400/8410 to draw up the specified amount of Internal Standard from the specified vial.
  • Page 54 Operation Default Clean Vial (I, II, III) – This parameter specifies the wash solvent vial that will be used by the Default Cleaning procedure. The default value is 1. Default Clean Volume (0-5, 0-10, or 0-100 µL) – This parameter defines the amount of cleaning solvent that will be drawn up and expelled to waste.
  • Page 55 Solvent Plug Selecting this softkey will display a page with the following parameters: Vial for Solvent Plug (I, II, III) – This parameter defines which of the vials will be used as the source for the solvent plug. The default selection is wash vial I.
  • Page 56 Operation Post-Injection Delay (0-99.9 sec) – Specifies the length of time the needle will remain in the injector after the syringes contents have been completely expelled. The default value is 0 sec. CP-8400 AutoSampler & CP-8410 AutoInjector...

  • Page 57: Instrument Menu

    Instrument Menu The Instrument Setup button displays a screen with two selections. The CP-8400/8410 selection has two screens that display the following parameters: Buzzer at Start of Cycle (On/Off) – A sound will be generated to warn of impending tower movements when this parameter is set to On. The default value is On.

  • Page 58: Cp-8400/8410 Status

    Operation Reset Plunger Stroke Counter (Softkey) – Selecting this softkey causes the Plunger stroke count to be reset to 0. Injector 1 Needle Depth (%) 0 to 100 default=100 Injector 1 Injection Speed (cm/sec) 1 to 30 default=30 Injector 2 Needle Depth (%) 0 to 100 default=100 Injector 2 Injection Speed (cm/sec)
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  • Page 60: Special Uses

    Special Uses The CP-8400 and CP-8410 have a variety of features that support novel uses for completing your analyses quickly and reliably. Some of the features include Dual and Duplicate injections, assigning analytical methods to certain vial ranges for “turn-key” type operation, the ability to meter Internal Standards with analytical injections and Priority Sample interruption of in-progress automation.

  • Page 61: Duplicate Run

    Duplicate Run Duplicate runs are performed exactly the same way as Dual runs except the “Advance Vial for second injection” parameter is set to No. This will cause the CP-8400/8410 to inject the contents of a single vial into both injectors.

  • Page 62: Automated Metering Of Internal Standard

    Special Uses Automated Metering of Internal Standard Internal Standard may be drawn up by the CP-8400/8410 syringe prior to drawing up the sample aliquot for injection. The Internal Standard may be placed into Solvent Vial positions I, II, or III. Internal Standard is always withdrawn before the sample to avoid contamination of the Internal Standard by sample that may remain on the syringe needle.

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