Agilent Technologies 6475 User Manual
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Agilent Technologies 6475 User Manual

Triple quad lc/ms system
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Agilent 6475 Triple Quad LC/MS System
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  • Page 1 Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 2 Copyright permitted by applicable law, Agilent hazard. It calls attention to an disclaims all warranties, either express or © Agilent Technologies, Inc. 2022 operating procedure, practice, or implied, with regard to this manual and any information contained herein, No part of this manual may be...

  • Page 3: Table Of Contents

    Instrument Status Indicators in Data Acquisition Modes of Operation ON mode OFF mode Standby mode Ion Sources Agilent Jet Stream (AJS) source Electrospray Ionization (ESI) source Atmospheric Pressure Chemical Ionization (APCI) source Multimode Ionization (MMI) source Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 4 Preparing the LC Modules Preparing the Triple Quad LC/MS Performing a Checktune or Autotune Switching LC stream to MS Monitoring MS baseline and spectral displays Viewing the system logbook for events and errors Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 5 Analyzing Data with Quantitative Analysis Maintenance Routine Tasks Calibrant Delivery System and Divert Value Sources Nebulizer Periodic Tasks Calibrant Delivery System and Divert Value Sources Nebulizer Corona Needle Ion Injector Rough Pump Disinfection Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 6: About This System

    About this System Sustainability Information 7 Agilent Regulatory Compliance Statement 8 Technical Support 13 Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 7: Sustainability Information

    Product End-of-Life Agilent offers a take-back program for its 6475 triple quadrupole LC/MS system customers in US, EU and UK markets. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 8: Agilent Regulatory Compliance Statement

    Declaration of Conformity. The Declaration of Conformity is the legal declaration by Agilent that the product complies with the relevant directives listed above, and shows the EN standards to which the product was tested to demonstrate compliance. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 9: Uk Compliance

    Declaration of Conformity. The Declaration of Conformity is the legal declaration by Agilent that the product complies with the relevant regulations listed above, and shows the designated standards to which the product was tested to demonstrate compliance. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 10: Electromagnetic Compatibility

    4 Make sure that all peripheral devices are also certified. 5 Make sure that appropriate cables are used to connect the device to peripheral equipment. 6 Consult your equipment dealer, Agilent Technologies, or an experienced technician for assistance. Changes or modifications not expressly approved by Agilent Technologies could void the user’s authority to operate the equipment.

  • Page 11: Emc Declaration For South Korea

    Sound Emission Certification for Federal Republic of Germany Sound pressure Sound pressure Lp < 70 dB(A) according to DIN EN ISO 7779. Schalldruckpegel Schalldruckpegel LP < 70 dB(A) nach DIN EN ISO 7779. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 12: Waste Electrical And Electronic Equipment (Weee) Directive

    The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not display of in domestic household waste. NOTE To return unwanted products, contact your local Agilent office or see https://www.agilent.com for more information. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 13: Technical Support

    To get answers to your questions, join over 10,000 users in the Agilent Community. Review curated support materials organized by platform technology. Ask questions to industry colleagues and collaborators. Get notifications on new videos, documents, tools, and webinars relevant to your work. https://community.agilent.com Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 14: Hardware Overview

    Hardware Overview 6475A LC/TQ 15 Instrument Status Indicator Lights 18 Instrument Status Indicators in Data Acquisition 19 Modes of Operation 20 Ion Sources 22 Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 15: 6475A Lc/Tq

    Hardware Overview 6475A LC/TQ 6475A LC/TQ Front view CDS bottle Ion source Status indicator Front cover Service panel Serial number Power switch Figure 1. Front view of 6475A LC/TQ Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 16: Calibrant Delivery System

    Hardware Overview Calibrant Delivery System Calibrant Delivery System LC flow inlet Selection valve Leak sensor CDS bottle Solenoid valve Figure 2. Calibrant delivery system Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 17: Side View

    Hardware Overview Side view Side view Circuit Remote breaker start 12.5 amp fuses (main electronic power supply) Line voltage Figure 3. Side view of 6475A LC/TQ Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 18: Instrument Status Indicator Lights

    Power Switch LED = Blinking Yellow Instrument State = BACKGROUND SCAN Instrument State = Off Status LED = Green Status LED = Off Power Switch LED = Green Power Switch LED = Off Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 19: Instrument Status Indicators In Data Acquisition

    ⯀Light Orange - Not Ready: The device is not ready ⯀Red - Instrument Error: The instrument has an error and cannot process samples. Blank - Disconnected: The connection to the instrument has been closed. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 20: Modes Of Operation

    ON mode. OFF mode When in the powered OFF state, the system is vented and the instrument is turned off to allow for maintenance or to prepare for long periods of non-use. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 21: Standby Mode

    • For the Agilent Jet Stream (AJS) source: The sheath gas temperature, drying gas flow, and drying gas temperature are reduced. • The spray chamber high voltages are turned OFF. • The mass spectrometer stop generating spectra. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 22: Ion Sources

    “Simultaneous ESI and APCI capability and high LC flow rate compatibility (up to 2 mL/min) means more compounds can be detected in less time with the Agilent Multimode source.” on page 26 For ion source maintenance, see “Sources” on page 62. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 23: Agilent Jet Stream (Ajs) Source

    ) is used to improve ion generation and desolvation. AJS source improves sensitivity 5x or more for many small- molecule compounds that undergo electrospray ionization. Figure 4. AJS cover, ion source, and spray shield Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 24: Electrospray Ionization (Esi) Source

    (desorbed) into the gas phase. These ions are attracted to and pass through a capillary sampling orifice into the mass analyzer. Figure 5. ESI cover, ion source, and spray shield Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 25: Atmospheric Pressure Chemical Ionization (Apci) Source

    Because high temperatures are also involved , APCI is not appropriate for analysis of large biomolecules. APCI is used with normal-phase chromatography more often than electrospray is because the analytes are usually nonpolar. Figure 6. APCI cover, ion source, and spray shield Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 26: Multimode Ionization (Mmi) Source

    Figure 7. MMI cover, ion source, and spray shield Simultaneous ESI and APCI capability and high LC flow rate compatibility (up to 2 mL/min) means more compounds can be detected in less time with the Agilent Multimode source. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 27: Tuning

    Calibrating the LC/TQ (Autotune) 31 Generating a Detailed Tune Report 32 Customizing the Actuals Window 33 Scheduling a Checktune 34 Scheduling an Autotune 35 Monitoring Baseline and Adjust Plot 36 Fixing Spray Stability EMF 37 Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 28: About Tuning

    15 to 20 minutes for each polarity. Everything is automatic since the tuning mix is delivered by the calibrant delivery system, which is switched on automatically during the tune. See “Calibrating the LC/TQ (Autotune)” page 31. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 29: Starting The Instrument

    On. You can also click the button in the title for the TQ Device Pane in the Instrument Status window. 4 Monitor baseline and adjust plot if necessary. See “Monitoring Baseline and Adjust Plot” on page 36. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 30: Calibrating The Lc/Tq (Checktune)

    Calibrating the LC/TQ (Checktune) A Checktune can be run with the following ion sources: ESI, AJS ESI, MMI, and APCI. The MMI source is only supported with the Ultivo and 6475 LC/TQs. Checktune is automatically run in both positive polarity and negative polarity.

  • Page 31: Calibrating The Lc/Tq (Autotune)

    Calibrating the LC/TQ (Autotune) An Autotune can be run with the following ion sources: ESI and AJS ESI. The MMI source is only supported with the Ultivo and 6475 LC/TQs. Autotune is automatically run in both positive polarity and negative polarity.

  • Page 32: Generating A Detailed Tune Report

    Generating a Detailed Tune Report A detailed tune report can be generated after you have run Autotune or Checktune. This is only supported on Ultivo and 6475 LC/TQs. 1 If necessary, open the Method Editor window. 2 Click the TQ tab.

  • Page 33: Customizing The Actuals Window

    6 Click OK when you finish selecting parameters. The parameters you selected appear in the Actuals window. Note that the customizations you have made will remain, even if you close the MassHunter Data Acquisition software and reopen it. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 34: Scheduling A Checktune

    Checktune. 5 Click the Scheduling slider to switch Scheduling on. 6 Select either Weekly or Monthly. 7 Select other options to indicate how often to schedule the Checktune. 8 Click Save. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 35: Scheduling An Autotune

    Autotune. 5 Click the Scheduling slider to switch Scheduling on. 6 Select either Weekly or Monthly. 7 Select other options to indicate how often to schedule the Autotune. 8 Click Save. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 36: Monitoring Baseline And Adjust Plot

    Click Freeze to temporarily stop the automatic scrolling of data along the x-axis. • Click Zoom Out to undo all zoom operations. • Click Undo Zoom to undo the last zoom operation. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 37: Fixing Spray Stability Emf

    “Calibrating the LC/TQ (Autotune)” on page 31. The spray stability is reevaluated as part of a tune. If it within the acceptable range, the EMF warning should be removed following completion of the tune. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 38: Basic Operation

    Edit DA Set up and run a Edit the LC Edit TQ method interactive parameters method parameters parameters samples Review Set up and results with Review results Quantitative Qualitative Analysis Analysis Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 39: Starting Up And Shutting Down The Instrument

    If you have any analyte in any of the components in the sample delivery system, flush the delivery system with pure solvent before you continue. 2 Check that the ballast valve on the rough pump is closed (if applicable). Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 40 6 Press the power switch located in the lower left front corner of the instrument. 7 Unplug the main power cable behind the service panel. This action prevents the turbo pump components from overheating. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 41: Starting The Data Acquisition Software

    Acq System Launcher icon in the system tray, and click Start Engines. If you have recently changed LC modules, remember to configure the instrument again. See the Installation Guide for instructions. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 42: Data Acquisition Windows

    Press F1 to obtain help on the active window. Drag a window border to resize the window. If you double-click the title of the window, the window “floats” outside of the main window. Double-click the title bar again to “dock” the window. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 43 This window displays the chromatogram plots in real time. These plots can be user-defined signals and/or instrument parameters. You select the plots in the Chromatogram tab in the QQQ tab in the Method Editor window. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 44 Specify an Override DA Method and select either Both Acquisition and DA or DA Only for the Method Type, and then Data Analysis is run as part of the method. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 45 Select whether to run Acquisition Only, to run Both Acquisition and DA, or to run DA only by selecting one of these options for the Part of method to run in the Worklist Run Parameters dialog box. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 46: Preparing The Lc Modules

    2 Purge the LC pump. Follow the directions for purging the pump in the User Guide for your pump. 3 Set up to condition or equilibrate the column. a Type LC parameters, and click Apply in the toolbar to download them to the LC. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 47 As you prepare for a run and during a run, you want to see the actual values of the instrument parameters. Do this in the Instrument Status window. a Right-click the Actuals list to see the Setup command. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 48 DAD, etc.) Set the background and text color for each parameter, and set a range for the parameters which are numbers. If the value of the parameter is not within the limits which you entered, then the background of the parameter is set to red. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 49 As you condition the column, you can set up the displays to monitor the effluent. a Right-click the chromatogram plot, and select Change. b In the Edit Signal Plot dialog box, select the type of display signal. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 50: Preparing The Triple Quad Lc/Ms

    If you did monitor the LC baseline with a VWD or DAD, you change back to the default instrument displays. 1 Right-click the chromatogram plot, and click Change. 2 Select the MS signal, and click OK. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 51: Viewing The System Logbook For Events And Errors

    When the System Logbook Viewer is open, you can select the time period to keep System Logbook entries. You can set the value from 1 week to 1 year. To do this, you click Tools > Purge Settings. The System logbook purge dialog box opens. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 52: Setting Up And Running An Acquisition Method

    Triggered MRM, you can copy and paste the transitions from the original Scan segments table to the Clipboard and then to the new Scan segments table. See the online Help for more information. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 53 To add a segment, click the Add Row or Insert Row icons. b Enter the parameters for each Scan segment. 5 Set up signals for the Chromatogram plot: a Click Chromatogram. b Select the Chromatogram Type, and type other plot values. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 54 Qualitative Analysis method that is connected, click browse to select a different method. When the Data Acquisition method is saved, the Qualitative Analysis method that you selected is copied or linked to the Data Acquisition method. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 55 If necessary, name the method and click OK. 10 Set up and run interactive samples: a Click the Sample Run window. b Enter the Sample Name, the Data File Name, the Path and other values. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 56 11 Set up and run worklists. a Click Worklist to show the Worklist window. b Click Add Multiple Samples . The Add Multiple Samples dialog box opens. c Enter all the information on the Sample Information tab. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 57 The DA part of this method is used instead of the DA part of the current method. You can also type the name of this method in the Add Multiple Samples dialog box. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 58: Reviewing Results With Qualitative Analysis

    • Select the most appropriate precursor and product ions for MRM analyses • Find compounds • Identify compounds • Do molecular feature extraction Refer to the online Help for the Qualitative Analysis software to learn more. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 59: Analyzing Data With Quantitative Analysis

    Refer to the online Help for the Quantitative Analysis program to learn how to do more operations to analyze your data. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 60 Calibrant Delivery System and Divert Value 66 Sources 67 Nebulizer 73 Corona Needle 78 Ion Injector 80 Rough Pump 83 Disinfection 89 When performing maintenance, it is recommended that you track procedures and details in a maintenance logbook. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 61: Maintenance Routine Tasks

    Enough tuning mix must be present to immerse the end of the intake tube. If the tuning mix level is within a few millimeters of the end of the intake tube, refill the calibrant bottle. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 62: Sources

    6 Dampen a clean lint-free cloth with a mixture of LC/MS-grade isopropanol and LC/MS-grade water. 7 Wipe the spray shield and the area around the spray shield. 8 Close the spray chamber. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 63 13 Add a 50:50 mix of LC/MS-grade isopropanol and LC/MS-grade water in a beaker. 14 Put the beaker in sonication for 5 minutes. 15 Drain the solvent and remove the parts from the beaker. 16 Dry the parts by using nitrogen gas. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 64 Do not hit tip of the corona needle. The tip can bend, which can decrease Clean and install the corona needle. C AU T I O N system performance. 25 Connect the nebulizer sample line and sample nebulizer gas tubing to the nebulizer. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 65: Nebulizer

    1 Install a mix of 30% to 70% methanol in water. On a monthly basis, prepare enough mix to flush overnight. 2 On a daily basis, flush for 3 minutes. On a monthly basis, flush overnight. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 66: Periodic Tasks

    The tuning mixes are not interchangeable. Failure to change the tuning mix C AU T I O N when the source or inlet assembly is changed can result in miscalibration of the instrument and errors in mass assignments. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 67: Sources

    The spray shield can be hot. Be careful not to burn yourself when you remove WAR N IN G the spray shield. 10 If you are changing to a different source type, remove the spray shield. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 68 The spray shield can be hot. Be careful not to burn yourself when you remove WAR N IN G the spray shield. 7 If you are changing to a different source type, remove the spray shield. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 69 Rinse a clean calibrant bottle with LC/MS-grade acetonitrile. b Pour the ESI Calibrant into the calibrant bottle. c Install the calibrant bottle into the calibrant delivery system. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 70 3 While you wait for the set points to equilibrate, prepare and install calibrant for the new source. a Rinse a clean calibrant bottle with LC/MS-grade acetonitrile. b Pour the APCI Calibrant into the calibrant bottle. c Install the calibrant bottle into the calibrant delivery system. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 71 3 While you wait for the set points to equilibrate, prepare and install calibrant for the new source. a Rinse a clean calibrant bottle with LC/MS-grade acetonitrile. b Pour the MMI Calibrant into the calibrant bottle. c Install the calibrant bottle into the calibrant delivery system. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 72 4 If the check tune results are not acceptable, run auto tune on a supported tuning source. 5 When the instrument is ready, load or create the method to use with the source. 6 Change the method settings for the new source. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 73: Nebulizer

    Tools needed: • Nebulizer Adjustment Fixture • Clean powder-free nitrile gloves • T6 Torx driver • 1/4-inch x 5/16-inch open-end wrench (Quantity: 2) Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 74 11 Turn on the LED Flashlight. The on/off switch is at the end of the flashlight. Do not hit or touch the end of the nebulizer or you can damage the needle. C AU T I O N Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 75 3 Install the nebulizer in the Nebulizer Adjustment Fixture. 4 Loosen the locknut next to the zero-dead-volume (ZDV) union. 5 Remove the union from the nebulizer needle holder. 6 Remove the union lock nut from the nebulizer. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 76 18 Insert the needle into the nebulizer shaft. 19 Adjust the electrospray needle position before reinstalling the nebulizer in the spray chamber. 20 Tighten the fastener (T6 Torx screw) that secures the needle holder in place. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 77 “Calibrating the LC/TQ (Autotune)” on page 31. The spray stability is reevaluated as part of a tune. If it within the acceptable range, the EMF warning should be removed following completion of the tune. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 78: Corona Needle

    5 Starting at the base of the needle, wipe the needle with a clean lint-free cloth. The cloth can be dry or dampened with LC/MS-grade isopropanol. 6 Reinstall the corona needle assembly in the spray chamber. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 79 Do not hit tip of the corona needle. The tip can bend, which can decrease C AU T I O N system performance. 7 Reinstall the corona needle assembly in the spray chamber. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 80: Ion Injector

    9 Loosen the collet clamping knob slightly to loosen the ion injector from the capillary puller tool. 10 Pull out the ion injector from the collet. 11 Unscrew the tool from the shield mount. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 81 8 Fill the graduated cylinder with deionized water and sonicate for 10 to 15 minutes. 9 Remove the ion injector from the graduated cylinder and remove the pipette tips (if any were used). 10 Blow out excess water from the ion injector bore with nitrogen. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 82 9 Lubricate the ion injector tip with LC/MS-grade isopropanol. 10 Install the ion injector cap over the outer end of the Ion injector. 11 Install the spray shield. 12 Close the spray chamber. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 83: Rough Pump

    WAR N IN G be dangerously hot. Never add or replace the rough pump oil while the pump is on. Hot oil can WAR N IN G splash out and cause harm. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 84 Do not fill the oil over the maximum mark beside the oil level window. C AU T I O N 8 Reinstall the fill cap. 9 Wipe off all excess oil around and underneath the pump. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 85 The back end of the filter is seated inside the filter mount. 13 Install the new large O-ring and filter cap. 14 Connect the exhaust tube. 15 Plug in the rough pump and all power cords from LC/MS instrument. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 86 3 Allow the rough pump to turn off automatically, this should take about 15 minutes. 4 Unplug all power cords from the LC/MS instrument. 5 Unplug the rough pump. 6 Remove first the fill cap and then open the drain plug. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 87 5 Close the lid. Make sure the O-ring remains properly seated to cover to achieve the required NOTE vacuum. The vacuum performance can be determined only after the pump starts. 6 Fasten the clamps. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 88 3 Using a flat-blade screw driver, remove the fuse holder of the blown fuse. 4 Replace with the appropriate fuse. 5 Reinstall the fuse holder. 6 Plug in the instrument. Agilent 6475 Triple Quad LC/MS System User Guide...

  • Page 89: Disinfection

    8 Allow all surfaces to completely air-dry. No moisture should be present on the instrument surfaces. 9 Discard the gloves and other personal protective equipment or clean them in an approved process, then wash your hands. Agilent 6475 Triple Quad LC/MS System User Guide...
  • Page 90  Agilent Technologies, Inc. 2022 October 2022 *D0025685* DocNo D0025685 DE22440912...

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