Load Kit; Transfer Metadata; Initialize Protocol On To Instrument; Start/Stop/Pause/Resume Protocol - Thermo Scientific Gibco CTS Rotea User Manual

The protocol checksum is an hexidecimal number with 32 characters.
Example: e152abb59e4949980731c5bbf7194ffb
It can be saved and compared as a string.
The ProtocolState report should now report ProtocolSelected.

Load kit

The instrument verification process needs the instrument door to be closed and a valid kit to be
detected. The kit ID is read by the instrument when the door is closed.
The DoorState is reported Closed, and the KitID string is supported by KitState report that checks the
kit type against that required for the protocol. If the kit ID is valid and aligned to the protocol then the
KitState is reported as valid.

Transfer metadata

Protocols can be defined with metadata or parameters that allow a qualified recipe to respond to
changing input materials and output targets.
Refer to the sequence diagram, see "MES drives Rotea in response to external systems"
After the protocol information is loaded the instrument will set ParameterName1 through
ParameterName20 to the names of the variables that need to set in the protocol metadata.
The MES can check ParameterValid1 to ParameterValid20 to determine the status of metadata
definition for the protocol. This will advise if that parameter is in use, if it has been set, and if the
setting is valid.
To set the value, use SetParameter1 through SetParameter20 and supply an Int32 value.

Initialize protocol on to instrument

Once the protocol has been specified, kit loaded and any metadata defined within acceptable ranges,
set the SetProtocolState to SendProtocolToRotea.
The protocol definition is sent to the instrument and ProtocolState will report ProtocolReadyToStart.

Start/Stop/Pause/Resume protocol

• The MES can Start, Stop and Pause/Resume the instrument with SetProtocolState variable.
• The ProtocolState will report ProtocolRunning.
• These actions are available through the buttons on the instrument when enabled.
• The MES can disable Start, Pause and Advance functions with the SetSkipButtonEnable and
SetPlayPauseButtonEnable. When the buttons are enabled they will follow the configuration in the
protocol file, if they are disabled the buttons on the instrument are disabled.
CTS ™ Rotea ™ Counterflow Centrifugation System User Guide
Chapter 4 OPC-UA interface
MES – Commands and reports overview
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