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Waters Prep 150 LC System Quick Start Manual
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Waters Prep 150 LC System Quick Start Manual

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Prep 150 LC System
Quick Start
715003940 / Revision B
Copyright © Waters Corporation 2014
All rights reserved
 

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Summary of Contents for Waters Prep 150 LC System

  • Page 1 Prep 150 LC System Quick Start 715003940 / Revision B Copyright © Waters Corporation 2014 All rights reserved  ...
  • Page 2 August 8, 2014, 715003940 Rev. B...
  • Page 3 We seriously consider every customer comment we receive. You can reach us at tech_comm@waters.com. Contacting Waters Contact Waters® with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. August 8, 2014, 715003940 Rev. B...
  • Page 4 34 Maple Street Milford, MA 01757 Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with.
  • Page 5 Consult Appendix A for a comprehensive list of warning and caution advisories. Operating the Prep 150 LC system When operating the Prep 150 LC system, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol...
  • Page 6 For compliance with the Waste Electrical and Electronic Equip- ment Directive (WEEE) 2012/19/EU, contact Waters Corpor- ation for the correct disposal and recycling instructions. Safety information Consult the operator's guides of the instruments or devices associated with this system for information about how to safely operate and maintain them.
  • Page 7 Preface This guide is part of the documentation set for the Waters Prep 150 LC system. It provides you with basic information about the major tasks you can perform with the Prep 150 LC system. For detailed information, refer to the Prep 150 LC system's Online Information System that you can access by clicking Help in the ChromScope software.
  • Page 8   Preface viii August 8, 2014, 715003940 Rev. B...
  • Page 9 Prep 150 LC System This quick-start guide presents nine elementary procedures that provide a basic knowledge of the Prep 150 LC system's operation and capabilities. Though relat- ively broad in scope, the guide offers enough instruction to allow purification of product compounds and collection of fractions.
  • Page 10 Prep 150 LC System Start the system To start the Prep 150 LC system and prepare it for operation, follow this proced- ure in conjunction with Good Laboratory Practice (GLP) guidelines and your labor- atory's standard operating procedures. Starting sequence: To start the system and prepare it for operation:  1.
  • Page 11 Start the system Unless the system administrator has disabled the System Requirement: Secure function, you must be a registered user and provide your user name and password, to start and log on to the ChromScope software.  4. Enter your user name and password, and then click Login, to start the ChromScope software.
  • Page 12  1. Inspect the fraction collection tubes and collection bed layout, to verify the module is ready to receive the run's fractions and waste.  2. Inspect the waste container, to ensure it can collect waste. Waters Fraction Collector III Operator's Guide. See also: Verifying operational readiness Optionally, run an instrument method using the Monitor control, to set initial conditions and verify the system's operational readiness.
  • Page 13 Set up your work environment Set up your work environment Before you begin using ChromScope software for the Prep 150 LC system to purify product and collect fractions, you can customize the system according to your pref- erences. These customizations become part of your user profile. As such, they appear whenever you log on to the system.
  • Page 14 Prep 150 LC System Task Description Set up your project You can create a new project or work with existing projects. folder. When you create a new project in the system, a project folder bearing the project name appears under the project's root node.
  • Page 15 Set up your work environment Task Description Decide how you You can select from among three file-open modes (New Tab, want to open and Current Tab, or Current Tab Overlay) according to how you work with the want to open and view data-log files. When you select a acquisition data-log mode, all data-log files you open remain open in that mode files.
  • Page 16 Prep 150 LC System Task Description   For Current Tab Overlay, the information in the data grid pertains to all of the data-log files opened in the current tab. Customize the In the Display Options window, you can specify and save...
  • Page 17 Quick Report to Printer", to generate a predefined report of your data. Import data from You can import data-log files (*.tta) from a defined location other Waters into a project's Data Files folder (or subfolder). This oper- products. ation is useful for importing scouting-run information used to set up the timing parameters for stacked-injection sample lists.
  • Page 18 Prep 150 LC System Verify the system and module settings Navigation tree > System Configuration > System Settings, Pump, Solvent Selector, Autosampler, and Detector tabs Navigation tree > System Status > Collection Bed Status To manage the system's operational readiness, you must define your preferences for shutting down the system in response to certain operating conditions, and for configuring the individual module's alert thresholds and usage settings.
  • Page 19 Verify the system and module settings To verify the system and module settings required for your configuration:  1. In the navigation tree, click the System Configuration node.  2. Click the tab for the system or module settings that you want to verify. To set up your solvent levels and warning and alarm thresholds, click Tip: the Solvent Selector tab, and configure the settings for each of your solvents.
  • Page 20 Prep 150 LC System Select and run a method-screening sample list To optimize collection of purified product, you must fine tune the purification chro- matography on the system using a scouting run, and store the optimal settings in a data-log file. To do so, you can select and run a method-screening sample list.
  • Page 21 Select and run a method-screening sample list Create a method-screening sample list using the wizard:  2. If you are using an autosampler, specify the location of the samples in the sample well-plate that you want to screen.  3. Select or create the instrument methods referenced in this method-screening sample list, ensuring the appropriate order of change for the samples and methods, which you select from separate lists.
  • Page 22 Prep 150 LC System  4. Load and run the method-screening sample list. Tip: During the scouting run, after you identify the optimum conditions for the run, to quit the remainder of the sample list, in the Main window, click "Skip to the next sample list"...
  • Page 23 Create and load the run's methods and sample list Create and load the run's methods and sample list Before you can collect fractions or purified product using the system, you must create the run's instrument methods, collection methods, and purification sample list.
  • Page 24 Prep 150 LC System For an integration method, you can specify the quantitation and identification parameters used to determine how you recognize and process a chromatogram's peaks. Later you can edit the method, as needed, and add it to a sample list before acquisition, or apply it to chromatograms after acquisition.
  • Page 25 Create and load the run's methods and sample list and then click the Sample List Wizard and Purification Sample List, to specify your remaining settings. Tips:   To set up a "run and report" queue, you can define and assign an integ- ration method and report template to the sample list's steps.
  • Page 26 Prep 150 LC System Start and monitor the run You start the run, and monitor its conditions and fractions, to purify compounds of interest and collect fractions. Monitor a run: To start the run and monitor its conditions and fractions:  1.
  • Page 27 Start and monitor the run queue is empty, the system immediately switches to system-shutdown conditions.   To manually collect fractions, also called emergency collection, from any of the Current Run sub-tabs, specify a starting location in the collection bed, and click Collection On.  ...
  • Page 28 Prep 150 LC System Analyze the run's data ChromScope software offers tools that quantitate the effectiveness of the methods, instrumentation, and processes that are used to collect purified product. Example chromatogram showing manual integration options: To analyze the purification run's data:  1.
  • Page 29 Analyze the run's data For more information, see these topics in the ChromScope online Help:   Open and view data-log files   Work with integration   Work with calibration   Test the purity of peaks detected by PDA   Interpret PDA peak purity results August 8, 2014, 715003940 Rev.
  • Page 30 Prep 150 LC System Report the results For verification of your run's results, you must create a report from the integrated data. To generate a basic, predefined report layout of your data, open the run's data-log file, and from the File menu, click "Print Quick Report to PDF" or "Print Quick Report to Printer".
  • Page 31 Report the results From the toolbar, click Print Quick Report To PDF Alternative:  4. In the PDF setting window, define a location in which to save the report, and click OK.  5. In the Save As dialog box, name the PDF file, and click Save. The message "Quick Report is not available on this Plot"...
  • Page 32 Prep 150 LC System Prepare the system for the next run or for shutting down Typically, you configure your purification sample lists and system inactivity options to prepare the system for the next sample run or shutdown period. System inactivity options:  ...

  • Page 33: Table Of Contents

    The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid August 8, 2014, 715003940 Rev.
  • Page 34 The following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices and on labels affixed to them or their compon- ent parts. Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. August 8, 2014, 715003940 Rev. B...

  • Page 35: Caution Advisory

      Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohaz- ards, corrosive materials, or toxic materials. To avoid personal contamination with biohazards, Warning: toxic materials, or corrosive materials, you must understand the hazards associated with their handling.
  • Page 36 A   Safety Advisories Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à...
  • Page 37 Warnings that apply to all Waters instruments and devices Warning: Use caution when working with any polymer tubing under pressure:   Always wear eye protection when near pressurized polymer tubing.   Extinguish all nearby flames.   Do not use tubing that has been severely stressed or kinked.
  • Page 38 A   Safety Advisories Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione:   Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati.   Spegnere tutte le fiamme vive nell'ambiente circostante.   Non utilizzare tubi eccessivamente logorati o piegati.  ...
  • Page 39 Warnings that apply to all Waters instruments and devices 경 고 : 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.   가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.   근처의 화기를 모두 끄십시오.   심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.  ...

  • Page 40: Warnings That Address The Replacing Of Fuses

    A   Safety Advisories Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L’utilisateur doit être informé que si le matériel est util- isé...
  • Page 41 Warnings that address the replacing of fuses If the fuse types and ratings appear on the instrument: Warning: To protect against fire, replace fuses with those of the type and rating printed on panels adjacent to instrument fuse covers. Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à...

  • Page 42: Electrical And Handling Symbols

    A   Safety Advisories If the fuse types and ratings do not appear on the instrument: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter. Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la...
  • Page 43 Electrical and handling symbols Alternating current Protective conductor terminal Frame, chassis, terminal Fuse Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Keep upright! Keep dry! Fragile!
  • Page 44 A   Safety Advisories August 8, 2014, 715003940 Rev. B...