Leica CV5030 Instructions For Use Manual page 94

8
Malfunctions and Troubleshooting
Problem
Air bubbles
(between
specimen and
cover slip).
Air bubbles
(between
specimen and
cover slip).
Air bubbles
(between
specimen and
cover slip).
Air bubbles
(between
specimen and
cover slip).
Air bubbles
(between
specimen and
cover slip).
Air bubbles
(between
specimen and
cover slip).
94
Possible cause
Dispenser needle is
bent.
Glued-on material
within the dispenser
group.
The dispenser
needle diameter has
not been selected
appropriately for the
coverslip mountant
used.
The air bubbles are
produced by leaks in
the hose system of
the dispenser group.
The coverslip
mountant is
incompatible with the
solvent used.
The suction cups
of the Pick & Place
module have become
stuck or deformed.
Remedy
1. Insert a new dispenser needle of the same size and
carry out a sufficiently long priming cycle before the
next coverslipping operation.
2. When reinserting the dispenser needle, always check
the needle height.
3. Check the dispenser needle cleaner: Fill this daily with
sufficient solvent. Replace the brush of the dispenser
needle cleaner if it has glued-on material and/or
hardening.
Glued-on material from hardened coverslip mountant in
the dispenser group usually cannot be seen. If air bubbles
continue to form despite carrying out the actions mentioned
above, rinse the dispenser group with 100 ml of a compatible
solvent. Do so in accordance with the instructions in
(→ p. 81 – 7. Cleaning and
The size of the dispenser needle has to be selected
according to the list of recommendations
5.12 Recommendation for parameter setting (beginning
with firmware 3.01.04))
or be determined as described in the configuration
recommendations.
If there is a leak in the hose system that cannot be remedied,
notify the responsible Leica Service.
Make sure that a solvent compatible with the coverslip
mountant is used in the loading bath of the CV5030 as well
as in the last steps of the preceding dyeing process.
If commercially available coverslip mountant is diluted with
a solvent that is incompatible, this can also cause small air
bubbles.
Incompatibility can frequently be identified by striation.
Non-functional suction cups impede coverslip placement.
Check the suction cups for glued-on material and
deformation and replace them if necessary.
If material has become glued-on because of incorrectly
configured parameters (such as too much coverslip
mountant), these have to be checked and readjusted.
Maintenance).
(→ p. 65 –
of the various coverslip mountants
Version 3.4, Revision M