Leica CV5030 Instructions For Use Manual
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Leica CV5030 Instructions For Use Manual

Robotic cover slipper
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Leica CV5030
Robotic Cover Slipper
Instructions for Use
English
Order No.: 14 0478 80101 - Revision M
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 3.4, Revision M - 04.2021

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  • Page 1 Leica CV5030 Robotic Cover Slipper Instructions for Use English Order No.: 14 0478 80101 - Revision M Always keep this manual with the instrument. Read carefully before working with the instrument. Version 3.4, Revision M - 04.2021...
  • Page 3 Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH.

  • Page 4: Table Of Contents

    Instrument Setup ....................... Installation site requirements ....................19 Unpacking the Leica CV5030 ....................20 4.2.1 Setting up the Leica CV5030 ..................... 22 Preparing and adjusting the instrument ..................22 4.3.1 Removing or installing the transport anchors ................23 Leveling the instrument ......................24 Exhaust system ........................
  • Page 5 5.13 Determing the optimum parameter setting (MENU A+B) ............. 68 5.13.1 Procedure ..........................68 Workstation operation ....................Operation as ST5010 — CV5030 workstation ................74 Operation as ST5020 — CV5030 workstation ................75 Important instructions for operation as workstation ..............78 Interrupting workstation operation ....................
  • Page 6 Table of Contents Appendix A — Application-related notes and recommendations ........12.1 Leica specimen slide holders, output and coverslip magazines made of plastic ......111 12.2 Specimen slide holders from other manufacturers ..............112 12.3 Specimen slides and gripper mechanism ................112 12.4 Leica CV5030 –...

  • Page 7: Important Information

    Symbols and their meanings Warning Leica Biosystems Nussloch GmbH assumes no liability for consequential loss or damage due to failure to observe the following instructions, particularly in relation to transportation and package handling, and failure to observe the instructions for handling the instrument carefully.
  • Page 8 Important Information Symbol: Title of the symbol: Manufacturer Description: Indicates the manufacturer of the medical product. Symbol: Title of the symbol: Manufacturing date Description: Indicates the date when the medical device was manufactured. Symbol: Title of the symbol: In vitro diagnostic medical device Description: Indicates a medical device that is intended to be used as an in vitro diagnostic medical device.
  • Page 9 Title of the symbol: This way up Description: Indicates correct upright position of the transport package. Symbol: Title of the symbol: Temperature limit for storage Description: Indicates the storage temperature limits to which the medical device can be safely exposed. Leica CV5030...

  • Page 10: Qualification Of Personnel

    Qualification of personnel • The Leica CV5030 may be operated by trained laboratory personnel only. • All laboratory personnel designated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it.

  • Page 11: Intended Use Of Instrument

    Important Information Intended use of instrument The Leica CV5030 is a robotic coverslipper for coverslipping tissue sections, cells or swabs mounted on specimen slides with cover slips made out of glass using a wide variety of different cover slip mountants.

  • Page 12: Safety

    To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings. Please note that electrostatic charge may result through direct or indirect contact with the Leica CV5030 Version 3.4, Revision M...
  • Page 13 • Never leave the instrument unattended for a long period of time. Particular care must be taken during a power failure to ensure that tissue sections do not dry out. • Remove all glass parts or other objects away from the Leica CV5030 working area during STOP. Only then may START be actuated.
  • Page 14 • Only Leica service technicians are authorized to open the instrument for maintenance and repair work. • When using cleaners, please comply with the safety instructions of the manufacturer and the laboratory safety regulations.

  • Page 15: Instrument Components And Specifications

    Height-adjustable instrument feet Output magazines Control panel Maintenance door Dispenser rest (park) position (prime position) Cover slip magazine Dispenser group Main switch Bottle for cover slip mountant Loading bath Pick&Place module (cover slip mount) Input door Unit cover Loading drawer Leica CV5030...

  • Page 16: Technical Data

    Instrument Components and Specifications Technical data Model name, model number Leica CV5030, 14 0478 39700 Nominal supply voltage: 100 – 240 V AC Nominal supply frequency: 50 – 60 Hz Power supply voltage fluctuations ± 10 % Power consumption: 100 VA Power input fuse: Thermal Circuit Breaker 5A (3120-…) Power supply: C14 inlet acc. to IEC 60320-1...

  • Page 17: Standard Delivery - Packing List

    Capacity of 20 or 30 specimen slides (up to 60 specimen slides) Standard delivery – packing list Order number The standard equipment for the Leica CV5030 includes the following parts: Basic instrument (local power cord included) 14 0478 39700 Dispensergroup, consisting of:...
  • Page 18 Exhaust hose, 3 m 14 0478 39820 Instructions for Use, printed (English), with Language CD 14 0478 80200 14 0478 80001 If the supplied local power cord is defective or lost, please contact your local Leica representative. Note Carefully check the delivery against the packing list and the delivery note. Should you find any discrepancies, please contact your Leica sales office without delay.

  • Page 19: Instrument Setup

    Instrument Setup Instrument Setup Installation site requirements The location for the Leica CV5030 Robotic Coverslipper must meet the following requirements: Warning • The installation location must be well ventilated, and must contain no sources of ignition of any kind. • The chemicals used in the Leica CV5030 are easily inflammable and pose a health hazard. • Never operate the instrument in rooms with an explosion hazard.

  • Page 20: Unpacking The Leica Cv5030

    If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. • Note this on the shipping documents and check the shipment for possible damage. • Only personnel authorized by Leica may unpack and install the instrument. Opening the package 1. Unscrew the 8 screws (→ Fig. 2-2) on the sides of the wooden box and loosen the cover.
  • Page 21 2. Unscrew 2 x 8 screws (front and rear on the instrument, (→ Fig. 4-1), loosen and remove the retaining clips (→ Fig. 4-2) from the baseplate. 3. Remove the dust cover from the instrument. For setting up the instrument, refer to (→ p. 22 – 4.2.1 Setting up the Leica CV5030). Fig. 4 Leica CV5030...

  • Page 22: Setting Up The Leica Cv5030

    Instrument Setup 4.2.1 Setting up the Leica CV5030 Setting up the Leica CV5030 1. Grab hold of bottom of the instrument from the front and rear (with at least 2 people; the instrument weighs approx. 57 kg) and place it on a stable laboratory table.

  • Page 23: Removing Or Installing The Transport Anchors

    3. Remove the transport anchor (→ Fig. 7-4) for the gripper. The gripper slowly moves downward in the process. 4. Using the provided No. 3 Allen key, unscrew the screws (→ Fig. 7-1) (→ Fig. 7-2) for the two red transport anchors (→ Fig. 7-3) and (→ Fig. 7-4): Leica CV5030...

  • Page 24: Leveling The Instrument

    Instrument Setup Fig. 7 5. To transport the instrument, reinstall the transport anchors in the opposite order. Note We recommend keeping the transport anchors and screws close to the instrument in the provided clear plastic bag. Leveling the instrument 1. Move the instrument to its final location on the work surface. When doing so, ensure that all four feet are on the work surface.

  • Page 25: Exhaust System

    • Additionally, safety instructions regarding the absence of voltage in the device as well as local laboratory regulations have to be observed. Fig. 9 4.5.1 Inserting the active carbon filter • Unscrew the slotted screw (→ Fig. 9-7) at the service door (→ Fig. 9-1) on the left side of the instrument and open the service door to the left. Leica CV5030...

  • Page 26: Attaching The Exhaust Hose

    Instrument Setup • Unscrew the screw (→ Fig. 9-2) of the filter cover (→ Fig. 9-3) using a No. 3 Allen key and pivot the cover upwards. • The insertion date can be written down on the adhesive label (→ Fig. 9-4). • Insert the filter (→ Fig. 9-5), close the cover (→ Fig. 9-3) and secure it back in place using the screw (→ Fig. 9-2).
  • Page 27 Make sure that the blue shutter ring is on the bottle neck and the O-ring (→ Fig. 10-6) is attached to the dispensing group (→ Fig. 10-5) correctly. 7. Finally, insert the cable and the air hose into the holder (→ Fig. 12-2) provided. Fig. 12 Leica CV5030...
  • Page 28 Instrument Setup Inserting the dispenser needle • Select the dispenser needle to be used for coverslipping from the scope of delivery (→ Fig. 14). • Insert the dispenser needle (→ Fig. 13-1) into the dispenser needle holder (→ Fig. 13-2) from below and turn the dispenser needle by 45° until one corner (→ Fig. 14-1) is over the retaining...

  • Page 29: Aligning The Dispenser Needle Height Relative To The Specimen Slide Outfeed

    • Do not cause the Pick & Place module to make any downward motion. 7. Now align the highest point of the specimen slide outfeed (→ Fig. 16-2) and the tip of the dispenser needle so that they are touching. Leica CV5030...

  • Page 30: Setting The Needle Height

    Instrument Setup Fig. 15 Fig. 16 4.7.2 Setting the needle height L The needle height has to be corrected after reinserting the dispenser needle. Version 3.4, Revision M...
  • Page 31 Fig. 17 Fig. 18 5. The dispenser is reset into the rest position if the 0 mm distance is set correctly. 6. Now carry out a three-quarters counterclockwise revolution using the No. 3 Allen key. Leica CV5030...

  • Page 32: Dispenser Needle Cleaner (Nozzle Cleaner)

    Instrument Setup 7. This achieves the optimum distance of 0.75 - 0.8 mm. 8. The set height of the dispenser needle can be inspected again by a visual inspection from the front (→ Fig. 16). 9. Then close the service door, screw it down tight and reconnect the instrument to the power supply.
  • Page 33 (→ Fig. 21-3). Make sure that the mounting bracket is parallel to the edge of the housing wall (ellipse, (→ Fig. 21)). Fig. 21 3. Then, fasten the holder (→ Fig. 22-3) on the mounting bracket using the screw (→ Fig. 22-1) and shim (→ Fig. 22-2). Leica CV5030...
  • Page 34 Instrument Setup 4. Insert the completely assembled dispenser needle cleaner (→ Fig. 22-4) into the holder as shown in (→ Fig. 22). Press down to ensure that the two lateral mounting clips (→ Fig. 22-6) audibly engage in the notches of the bracket. Fig. 22 Alignment to the dispenser needle 1.

  • Page 35: Connecting The Power Supply

    Connecting to power supply 1. Make sure that the coverslipping machine is switched off: The power switch (→ Fig. 25-3) the front side must be in the "0"= position. 2. Insert a suitable power cable into the power input socket (→ Fig. 25-1). Leica CV5030...

  • Page 36: Installing The Accessories

    Note There are two interface ports: SERIAL port (→ Fig. 47-3) has two functions. On the one hand this RS 232 port serves as an intercommunication interface to either the Leica ST5010 or the Leica ST5020 via the transfer station Leica TS5025. On the other hand it serves as service interface. For you as a customer only the first purpose is allowed for use. RS 485 port (→ Fig. 47-5) serves a pure service interface. Customers are not allowed to use it for any purpose.
  • Page 37 • Fill the output station (→ Fig. 28-2) with the desired number of output magazines (→ Fig. 28-1). Up to two size 30 output magazines or three size 20 output magazines fit in the output station. • Operating the instrument requires that at least one output magazine is inserted. Fig. 28 Leica CV5030...

  • Page 38: Refilling Consumables

    (→ Fig. 29-3). • The standard scope of delivery includes the bath insert for the Leica 30 specimen slide holder. The respective associated insert must be used when using other specimen slide holders (Leica metal 20 specimen slide holder,...
  • Page 39 O-ring is present and positioned correctly on the dispensing group and the mountant bottle's blue shutter ring. Warning Only glass bottles provided by Leica may be used (see (→ p. 99 – 9. Optional Accessories) (→ p. 17 – 3.3 Standard delivery – packing list).

  • Page 40: Operation

    Operation Operation Control panel functions Note (→ Fig. 31) is provided as an example only and shows a valid start display for this instrument. Fig. 31 The control panel field consists of a membrane keyboard with ten pushbuttons, four LED indicators and a two-line LCD display. • It is used to control the functions of the instrument and to display the software status. • The control panel field indicates the current status of the robotic coverslipper and operations in progress.

  • Page 41: Key Functions For Instrument Operation

    After the output magazine reaches the output position, all specimen slides must be removed before the coverslipping operation can be resumed. Non-observance can lead to damage of the specimen slide. RESPOND A signal tone can be acknowledged using the RESPOND button. Leica CV5030...

  • Page 42: Switching The Instrument On Or Off

    Operation RELEASE COVER SLIP RELEASE COVER SLIP ventilates the Pick & Place module's suction cups. The cover slip is released. The function is disabled while the BUSY LED is illuminated in green. RELEASE SLIDE Pressing RELEASE SLIDE opens the gripper jaws as long as the button is held down.
  • Page 43 The message disappears and the instrument is ready to operate. The displays shows the message READY. If the READY LED switches from red to green, the coverslipping operation can be started by pressing the START button. Leica CV5030...

  • Page 44: Brief Inspection Before Starting The Coverslipping Operation

    5.4.1 Bath insert for HistoCore SPECTRA ST Note The Bath Insert and Clip is required to to be used with the HistoCore SPECTRA ST specimen slide holder for 30 specimens on the Leica CV5030 Robotic Coverslipper. Version 3.4, Revision M...
  • Page 45 (→ Fig. 35-1) has to be removed from the specimen slide holder before inserting it into the Leica CV5030. Preparing the Leica CV5030 for the HistoCore SPECTRA ST Bath Insert: 1. Open the load drawer of the Leica CV5030 (→ Fig. 32-4) and pull the loading bath forward.

  • Page 46: The Coverslipping Operation

    (→ Fig. 35). 6. Remove the specimen slide holder handle (→ Fig. 35-1). Fig. 35 7. Open the load drawer of the Leica CV5030 and insert the specimen slide holder into the HistoCore SPECTRA ST Bath Insert. 8. Cover slip the specimen slides (→ p. 46 – 5.5 The coverslipping operation).
  • Page 47 • If some or all specimen slides are to be removed, use the LIFT key to raise the output magazines to the removal position. Removal is not absolutely necessary after each specimen slide holder, as long as sufficient capacity remains in the output magazine for the next coverslipping cycle (max. capacity = 60 specimen slides). Leica CV5030...

  • Page 48: Interrupting The Coverslipping Operation

    Operation Note If the space in the output magazine has already been assigned completely but there are still SPECIMEN SLIDES TO BE COVERSLIPPED present in the loading bath, this is indicated by the message PAUSED CHECK LIFT accompanied by a signal tone. CHECK LIFT message is a note that the assigned output magazine has to be removed.
  • Page 49 • Confirm the signal tone by pressing the RESPOND button. Warning Remove all glass parts or other objects away from the Leica CV5030 working area after pressing STOP. Only then may START be actuated. • Using the RELEASE COVER SLIP and RELEASE SLIDE buttons, you can release any cover slip that has been picked up or specimen slide that has been gripped.
  • Page 50 Operation • Only press the START button to continue working after doing so. The instrument then initializes. • Continue as outlined in (→ p. 42 – 5.3 Switching the instrument on or off). Warning The output magazines are moved to the output position automatically after pressing START.
  • Page 51 2. Whether the distances between the individual specimen slides are correct. 3. Whether the specimen slide is damaged. • Consider moving the ungripped specimen slide forward by one position in the specimen slide holder or removing it and applying a cover slip by hand. Leica CV5030...
  • Page 52 Operation • If everything is in good condition, work can be continued, as in normal PAUSE mode, by pressing START. Warning Specimen slides that do not conform to ISO 8037/1 are not detected by the instrument and have to be coverslipped by hand.

  • Page 53: Display Indicators And Instructions

    Display indicators and instructions Note The display of the Leica CV5030 is used to display status and error messages that provide information about the current status of the instrument. Status messages always appear in the bottom line of the display. The top line displays the operating status of the instrument. The following table compiles all status messages.
  • Page 54 Operation Display Explanation Action The user is prompted to Open the loading door, pull out the check the loading bath. loading drawer and loading bath. Then check whether there is a lid on the loading bath or an empty rack inside it (remove both if they are present).
  • Page 55 Check the cover slips in the cover row are defective. slip magazine. If the messages occurs repeatedly despite the cover slips being intact, clean the cover slip sensor. If the message persists, clean the Pick & Place module (skids and suction cups). Leica CV5030...
  • Page 56 Operation Display Explanation Action The user has pressed the The output magazine, if loaded, has STOP button. to be removed from the instrument. Grabbed specimen slides or held cover slips also must be removed from their position by pressing the respective RELEASE SLIDE or RELEASE COVERSLIP button.
  • Page 57 Note The following status messages are displayed only if the instrument is connected to a staining machine (Leica ST5010 AutoStainerXL or Leica ST5020 Multistainer) via a transfer station. Indicates that a specimen Wait for the specimen slide to finish slide holder that has been processing.

  • Page 58: Button Functions For Programming

    Leica CV5030. Button functions for programming Press and hold for 2 s Press and hold RESPOND for approx. two seconds to set the programs and parameter sets the instrument uses to operate.

  • Page 59: Setting Parameter Sets

    • Pressing the START button calls up the next higher program. • Pressing the PAUSE button calls up the next lower program (→ Fig. 39). Fig. 39 5.10 MENU A — parameter settings L The individual buttons used to select and program the respective parameters are in the bottom row of the control panel field. Leica CV5030...
  • Page 60 Operation Example: 1. To modify the length of the coverslip mountant stroke (STK), press the RELEASE COVERSLIP button (→ Fig. 40). 2. The name of the parameter and its currently set value appear on the display (→ Fig. 40-1). The selected program (SET 1 through 4) is shown in the top right.
  • Page 61 1 = less coverslip mountant to 9 = maximum quantity of coverslip mountant • The quantity of the coverslip mountant applied also depends on the composition of the mountant (e.g. viscosity) and the selected dispenser needle (different diameters). Mountant Type — dispensing pressure Leica CV5030...
  • Page 62 Operation The pressure needed to dispense the coverslip mountant is set in increments of 1: 1 = 100 mbar/lowest pressure to 10 = 1000 mbar/highest pressure • An increase of "1" increases the pressure by 100 mbar. • The pressure has to be adapted based on the coverslip mountant used (different viscosities) and the dispenser needle used (different diameters).

  • Page 63: Menu B - Parameter Settings

    • If the starting point for the coverslip mountant stroke is to be moved towards the label field, a positive value between +10 and +100 has to be selected. • The changes can be made in increments of 10. One increment corresponds to 1.0 mm. • The factory preset value is 0. Fig. 44 Leica CV5030...
  • Page 64 Operation Speaker Volume Level — volume of the audible signal Setting for the volume of key tones, messages and the signal tone: In increments of 1 from (quiet) to (loud) Note The signal tone and audible signals of the buttons cannot be disabled completely. Coverslip Limit Adjustment —...

  • Page 65: Leaving The Parameter And Submenu

    5.12 Recommendation for parameter setting (beginning with firmware 3.01.04) The following settings serve as recommendations and have to be adapted to the conditions in the respective lab. The parameter settings have been determined as follows under standardized condition in a Leica Biosystems Nussloch GmbH lab: • Use of tissue sections with a thickness of 3-5 μm.
  • Page 66 They have to be adapted on a case-by-case basis. Note Recommendations for settings for earlier firmware versions (before firmware version 3.01.04) can be requested from your local Leica Biosystems representative. We recommend using only consumables validated by Leica! Version 3.4, Revision M...
  • Page 67 Fluka Xylene Pertex Medite Xylene Mounting Richard-Allen Xylene Medium No. Scientific 4111/4112 Cytoseal Xyl Richard-Allen Xylene Scientific Cytoseal 60 Richard-Allen Xylene (280) Scientific Eukitt O. Kindler Xylene Histofluid Marienfeld Xylene Consul-Mount Shandon/Thermo Xylene / Histology Scientific Formulation Leica CV5030...

  • Page 68: Determing The Optimum Parameter Setting (Menu A+B)

    Operation Designation Manufacturer/ Loading bath Dispenser Type supplier filled with needles Consul-Mount Shandon/Thermo Xylene / Cytology Scientific Formulation Shandon Shandon/Thermo Xylene Xylene Scientific Substitute Mountant Histokitt II Carl Roth GmbH Xylene Aquatex: Merck KGaA Distilled water STP: (water based) 40/50 CSP: varies 5.13 Determing the optimum parameter setting (MENU A+B) L The following procedure provides assistance for:...
  • Page 69 • Fill the dispenser needle cleaner, being mindful of the correct seating (→ p. 32 – 4.8 Dispenser needle cleaner (nozzle cleaner)). • Fill the loading bath with a suitable solvent (→ p. 38 – 4.11 Refilling consumables). • Fill the cover slip magazine (→ p. 36 – 4.10 Installing the accessories). Leica CV5030...
  • Page 70 Operation 5. Important information regarding test run procedure for determining the optimum coverslipping parameters Note • The initial coverslipping trials should be carried out with empty specimen slides, i.e. without a specimen. • If coverslipping quality is good, subsequently carry out a test series using an applied specimen. • Since factors such as section thickness and specimen composition and type can affect the coverslipping quality, it may be necessary to make minor corrections to the parameter settings.
  • Page 71 8. Fine-tuning the adhesive and coverslip support positioning Here, the following parameters must be matched to each other: (Stroke position - starting point for the coverslip mountant stroke) (Cover slip placement position) and (Stroke length correction for the coverslip mountant stroke) Leica CV5030...
  • Page 72 Operation Fig. 45 Label field = STROKE LENGTH CORRECTION (length correction for the coverslip mountant application) = MOUNTANT STROKE (length of the coverslip mountant application) = STROKE POSITION CORRECTION (starting point of the coverslip mountant application) = COVERSLIP POSITION CORRECTION (cover slip placement position) •...
  • Page 73 LEN. The application quantity of the coverslipping mountant remains constant during this process. Only the length of the strip of coverslip mountant is shortened. This also makes it possible to correct small bubbles in the area of the coverslip edge near the label field. Leica CV5030...

  • Page 74: Workstation Operation

    STAINER PROC. appears on the display. Fig. 46 • Afterwards, the specimen slide holder is transfered to the Leica CV5030, placed in the loading bath and processed. After the coverslipping operation, the empty specimen slide holder is transferred back onto the discharge chute of the transfer station.

  • Page 75: Operation As St5020 - Cv5030 Workstation

    Transfer from the slide stainer to the robotic coverslipper is now possible once again. Manual loading Warning Basically, we do not recommend manual loading of the Leica CV5030 in workstation operation, as this can impair or stop the program flow of the Leica ST5010 AutoStainerXL. • However, if it is necessary to insert a specimen slide holder into the loading bath manually for coverslipping, the empty specimen slide holder must also be removed from the loading drawer manually.
  • Page 76 • In workstation mode, the robotic coverslipper must be ready to start (READY) so that the specimen slides can be processed properly. • If the Leica CV5030 is not ready to start and requires user intervention, this is indicated by an alarm message on the screen of the Leica ST5020 Multistainer, accompanied by an alarm sound.
  • Page 77 • If the discharge chute is already occupied with two empty specimen slide holders, an additional TS FULL message is shown on the display of the Leica CV5030. • With a third specimen slide holder, the discharge chute is completely occupied and the robotic coverslipper emits a signal tone, accompanied by the messages TS FULL...

  • Page 78: Important Instructions For Operation As Workstation

    Workstation operation Warning Basically, we do not recommend manual loading of the Leica CV5030 in workstation operation, as this can impair or stop the program flow of the Leica ST5020 Multistainer. • If it is necessary to insert a specimen slide holder into the loading bath manually for coverslipping, the empty specimen slide holder must also be removed from the loading drawer manually. The empty specimen slide holder is not conveyed automatically into the transfer station and thus onto the discharge chute.
  • Page 79 The default specimen slide holders intended for workstation operation are Leica plastic holders for 30 specimen slides (Order No. 14 0475 33643). Use of other Leica specimen slide holders (e.g. Leica plastic holders for 20 specimen slides) or specimen slide holders from other manufacturers is not permissible in workstation operation.

  • Page 80: Interrupting Workstation Operation

    Workstation operation Interrupting workstation operation • The PAUSE and STOP buttons have mostly the same functions outlined in (→ p. 48 – 5.6 Interrupting the coverslipping operation). • If the coverslipping operation is interrupted using the STOP button, the coverslipper must be initialized. To do so, press the START button. • After the coverslipping operation is interrupted using the STOP button, the specimen slide holder, which is still in the instrument, must be removed from the loading bath manually after processing is finished. In this case, it...

  • Page 81: Cleaning And Maintenance

    • Clean the cover slip sensors, suction cups, skids of the Pick & Place module, transport chain, specimen slide holder and output magazines using a lint-free cloth soaked in compatible solvent. • Never leave accessories lying in solvent or water for a long time (e.g. overnight) to prevent damage. Leica CV5030...

  • Page 82: Daily Cleaning And Maintenance - Overview

    Cleaning and Maintenance Daily cleaning and maintenance - overview Check the loading chute and complete working area of the transport chain for broken glass and adhesive residue. Carefully remove the adhesive residue and broken glass (→ p. 84 – 7.5.1 Loading chute and bath transport with transport chain).

  • Page 83: Cleaning And Maintenance As Necessary

    (→ p. 89 – 7.7.3 Transfer arm of the TS5015 or TS5025 Transfer Station for workstation operation) and carriage in y-direction. • TS5025: Discharge chute (→ p. 88 – 7.7.2 Discharge chute of the TS5015 or TS5025 Transfer Station for workstation operation) and transfer arm. Leica CV5030...

  • Page 84: Description Of The Required Daily Cleaning Measures

    Cleaning and Maintenance Description of the required daily cleaning measures 7.5.1 Loading chute and bath transport with transport chain • Check the loading chute and bath transport with transport chain for broken glass and adhesive residue and carefully remove any dirt or debris. Warning There is a danger of cutting injuries during this cleaning step. Therefore, proceed with the necessary caution.

  • Page 85: Skids Of The Pick & Place Module

    • The cover slip sensor is located on the bottom of the Pick & Place module, between the two suction cups. The cover slip sensor can be moved freely if it can be moved up and down by tapping it gently with the fingertip. Leica CV5030...

  • Page 86: Specimen Slide Outfeed

    Cleaning and Maintenance • If the cover slip sensor cannot be moved and/or you can feel glued-on material, carefully clean the sensor using a lint-free cloth soaked in compatible solvent. Fig. 52 7.5.11 Specimen slide outfeed • Check the specimen slide outfeed for adhesive residue and, if necessary, clean it carefully using a lint- free cloth soaked with compatible solvent.

  • Page 87: Dispenser Needle Cleaner (Nozzle Cleaner)

    • Check the output magazines for clinging and dried coverslipping mountant. • In particular, dried adhesive residue in the slots of the output magazines can cause problems when inserting the specimen slides. • Carefully clean the output magazines using a lint-free cloth soaked in compatible solvent. Leica CV5030...

  • Page 88: Description For Cleaning And Maintenance As Necessary

    Cleaning and Maintenance Warning Notice: The output magazines must not be placed in solvent over a period of multiple hours (e.g. overnight) (→ p. 81 – 7.1 Notes on cleaning and maintenance). Description for cleaning and maintenance as necessary 7.7.1 Active carbon filter Warning Active carbon filter not handled appropriately Serious injury, instrument damage, threat to environment...

  • Page 89: Transfer Arm Of The Ts5015 Or Ts5025 Transfer Station For Workstation Operation

    (→ p. 82 – 7.3 Weekly cleaning maintenance). • Insert a mountant bottle with the new mountant. • Run a prime cycle. Hold down the PRIME button until the hose is completely free of air bubbles and filled with coverslip mountant. Leica CV5030...

  • Page 90: Changing From A Xylene Substitute Coverslip Mountant To A Xylene-Based Mountant

    Cleaning and Maintenance 7.8.2 Changing from a xylene substitute coverslip mountant to a xylene-based mountant Warning The sequence of individual substances has to be followed to avoid milky/cloudy clumping in the hose and the dispenser group. • Remove the mountant bottle. • Insert a second glass bottle with 150 ml of xylene substitute and rinse/prime it (→...

  • Page 91: Malfunctions And Troubleshooting

    GR-Z BLOCKED use RELEASE SLIDE to open the gripper tongs and manually remove the specimen slide. Then switch the instrument off and on and proceed as described in (→ p. 42 – 5.3 Switching the instrument on or off). Leica CV5030...
  • Page 92 Malfunctions and Troubleshooting Display Cause Troubleshooting Error 312 The transfer arm of the Remove the cause of the blockage (specimen TS5025/5015 Transfer slide holder) and inspect the transfer arm of TS-X BLOCKED Station is blocked in its left/ the TS5025/TS5015. Remove empty specimen right movement (X-axis).

  • Page 93: Troubleshooting

    CS SENSOR DEF. in compatible solvent as described in (→ p. 85 – 7.5.10 Cover slip sensor). Error 322 The reference values of the Contact Leica Service. instrument are faulty. CONFIG FAULT Troubleshooting Problem Possible cause Remedy...
  • Page 94 The coverslip Make sure that a solvent compatible with the coverslip (between mountant is mountant is used in the loading bath of the CV5030 as well specimen and incompatible with the as in the last steps of the preceding dyeing process.
  • Page 95 (→ p. 29 – 4.7 Aligning applied. inserted incorrectly. the dispenser needle height relative to the specimen slide outfeed). Note Notice! Make sure the dispenser needle is securely in place (clamp is in the retaining plate). Leica CV5030...
  • Page 96 Warning Attention! If the actions described above do not remedy the problem that has occurred, contact the responsible Leica Service. The cover slips 1. The cover slips 1. The cover slips have to be flush against the edge of the have been have been front side in the coverslip magazine.
  • Page 97 Notice! When using the deeper loading bath in them. workstations (Leica ST5010 AutoStainerXL or Leica ST5020 Multistainer), take into account that a corresponding technical modification is necessary when using older models or upgrading a workstation. Contact the responsible Leica service for this purpose.
  • Page 98 Clean the transport area with a suitable solvent, such as xylene. If the chain mechanism does not function even after being cleaned, contact the responsible Leica Service. Version 3.4, Revision M...

  • Page 99: Optional Accessories

    Varistain adapter for Varistain 24-2 14 0464 37659 Adapter for DRS601/Varistain XY 14 0464 37058 Bath insert for Leica specimen slide holder for 30 specimen slides 14 0478 39593 Bath insert for Leica specimen slide holder for 20 specimen slides 14 0478 36706...
  • Page 100 Xylene-free. Note Leica provides no warranty for the function of specimen slide holders from other manufacturers or any warranty for their function in the instrument. The instrument operator bears responsibility for any use of specimen slide holders from other manufacturers!
  • Page 101 (→ Fig. 59-8) • 1 mounting bracket (→ Fig. 59-4) with 2 hexagon socket screws (→ Fig. 59-9) • 1 hexagon spanner (→ Fig. 59-6) • 1 plastic pipette (7.7 ml) (→ Fig. 59-5) Fig. 59 • 1 set of Instructions for Use Order No. 14 0478 40941 Leica CV5030...
  • Page 102 Optional Accessories Dispenser needle cleaner, small Set consisting of: • Container • Cover • Brush Order No. 14 0478 40559 Fig. 60 Dispenser brush Set of 5 Order No. 14 0478 41115 Fig. 61 Cover slip catch tray Order No. 14 0478 39585 Fig. 62 Output magazine 30, for 30 specimen slides, 4 pcs.
  • Page 103 Cover for loading bath Order No. 14 0478 39584 Fig. 67 Cover with cutout, for loading bath, only together with bath insert for Leica specimen slide holder for 30 specimen slides - 14 0478 39593 Fig. 68 Order No. 14 0478 40337 Leica CV5030...
  • Page 104 Optional Accessories Glass bottle with cover, bottle for coverslipping mountant, volume 250 ml, empty, with cover Order No. 14 0464 36537 Fig. 69 Cover slip magazine, coverslip magazine Multi-size with inserts for holding various common coverslip sizes 40-60x22 mm Order No. 14 0478 39748 40-60x24 mm Fig. 70...
  • Page 105 Optional Accessories Glass vial with cover Order No. 14 0478 39789 Fig. 72 Suction cups, pack of 2 Order No. 14 0478 39701 Fig. 73 Leica CV5030...
  • Page 106 Optional Accessories Kit Adapter Rack 30 HistoCore SPECTRA ST, for Leica specimen slide holders for 30 specimen slides. Consisting of: 1 Adapter 1 Clip Fig. 74 Order No. 14 0478 55522 Kit Clip Rack 30 HistoCore SPECTRA ST, Consisting of 2 Clips Order No.
  • Page 107 (14 0464 37659) Order No. 14 0478 38029 Fig. 77 Varistain adapter, Adapter for Shandon Varistain 24-4, Note For use with Leica specimen slide holder 30, modified, plastic 14 0478 38029, to combine Varistain 24-4 with Leica CV5030. Order No. 14 0464 37659 Fig. 78 Leica CV5030...
  • Page 108 Optional Accessories Bath insert Leica 20, for Leica specimen slide holders, for 20 specimen slides Fig. 79 Order No. 14 0478 36706 Bath insert Sakura 20, for Sakura specimen slide holders, for 20 Fig. 80 specimen slides Order No. 14 0478 36707...

  • Page 109: Warranty And Service

    Service information If you are in need of technical customer support or spare parts, please contact your Leica representative or the Leica dealer where you purchased the instrument. Please provide the following information: • Model name and serial number of the instrument.

  • Page 110: Decontamination Confirmation

    Decontamination Confirmation 11. Decontamination Confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decontamination confirmation on our website www.LeicaBiosystems.com within the product menu. This template has to be used for gathering all required data. When returning a product, a copy of the filled and signed confirmation has to be enclosed or passed on to the service technician. The responsibility for products that are sent back without this confirmation or with...

  • Page 111: Appendix A - Application-Related Notes And Recommendations

    • Coverslip magazine for cover slips 40-60x24 mm (14 0478 39749) • Coverslip magazine for cover slips 40-60x22 mm (14 0478 39748) Note Never store Leica plastic accessories for a long time (for example, overnight for cleaning purposes) in solvent or at high temperatures! For the output magazines to function flawlessly, avoid deformation of the side panels of the output magazines.

  • Page 112: Specimen Slide Holders From Other Manufacturers

    Appendix A — Application-related notes and recommendations 12.2 Specimen slide holders from other manufacturers Leica provides no warranty for the function of specimen slide holders from other manufacturers or any warranty for their function in the instrument. The instrument operator bears responsibility for any use of specimen slide holders from other manufacturers.

  • Page 113: Leica Cv5030 - Validated And Recommended Specimen Slides

    The following specimen slides were validated for the specimen slide gripper starting with serial number 3000: Note Leica provides no warranty for the function of specimen slides from other manufacturers or any warranty for their function in the instrument. The instrument operator bears responsibility for any use of specimen slides from other manufacturers.

  • Page 114: Cover Slips

    The Universal Label labels are optimized for use on the Leica CV5030 coverslipper and provide a complete solution for printing and coverslipping in combined usage. To ensure the best print quality, Leica Universal Label labels have to be used on the Cognitive Label printer. This is available exclusively from Leica Biosystems.
  • Page 116 Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 - 19 69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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