Leica CV5030 Instruction Manual
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Leica CV5030 Instruction Manual

Robotic coverslipper
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Leica CV5030
Robotic Coverslipper
Instruction Manual
Leica CV5030 - Robotic Coverslipper
V1.1 English - 11/2002
Always keep the manual near the instrument!
Read it carefully prior to operating the instrument!

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Summary of Contents for Leica CV5030

  • Page 1 Leica CV5030 Robotic Coverslipper Instruction Manual Leica CV5030 - Robotic Coverslipper V1.1 English - 11/2002 Always keep the manual near the instrument! Read it carefully prior to operating the instrument!
  • Page 3 Leica reserves the right to change technical understand it following thorough investigation specifications as well as manufacturing pro- in this field.

  • Page 4: Table Of Contents

    Interrupting the coverslipping process ..................26 Process-based interruptions ......................27 Operating the CV5030 in connection with the TS5025 transfer station ..........30 Differences compared to the CV5030 stand-alone operation ........... 30 Requests by the robotic color multistainer ST5020 ..............30 Interruption by the user ........................

  • Page 5: Important Information

    1. Important information Symbols in this manual and their Designated use meaning The Leica CV5030 is a robotic coverslipper for Caution notes coverslipping tissue sections, cells or swabs are highlighted in gray and marked by mounted on slides with coverslips made out of warning triangle.

  • Page 6: Safety

    The protective devices located on the unit and the accessories must not be removed or modified. The unit must only be opened and repaired by service technicians authorized by Leica. Instruction Manual V 1.1 - 11/2002...

  • Page 7: Caution Notes

    In this case, a two-hour waiting period must be maintained before switching on the unit. If the waiting period is not maintained, the unit may be damaged. The device must be aligned perfectly before startup. Leica CV5030 - Robotic Coverslipper...
  • Page 8 Safety Operating the unit The unit may only be operated by trained laboratory personnel. It may only be used according to its intended use and the instructions in this instruction manual. Avoid skin contact with solvents or coverslip media. Ensure correct dosage of mounting media. Excessive media may spill from the specimen slide onto the working surface and thereby obstruct the movement of parts of the unit (notes on cleaning).

  • Page 9: Unit Components And Specifications

    Unit components and specifications Overview—Components / functions Unit cover Output rack guide Bottle with mountant Coverslip magazine Dispenser park position Control panel Maintenance display door Fig. 1 Leveling feet On/Off switch Input door Leica CV5030 - Robotic Coverslipper...

  • Page 10: Functions Of The Control Panel

    Unit components and specifications Functions of the control panel LCD display LED READY LED BUSY LED PAUSE Extract LED ERROR output rack RESPOND/Acknowl- edging an audible alarm STANDBY RELEASE COVERSLIP Fig. 2 RELEASE SLIDE PRIME/ VENT MOUNTANT/ Open dispens- Bottle/Vent the bottle ing valve with mountant Instruction Manual V1.1 - 11/2002...

  • Page 11: Scope Of Delivery

    Unit components and technical data 3.3. Scope of delivery The basic unit is operational without additional accessories. The standard equipment of Leica CV 5030 consists of: • 1 basic unit, • 1 accessory kit consisting of: • 1 slide rack 30, plastic, pack of 5, •...

  • Page 12: Technical Data

    Dimensions and weight: Width: 420 mm Depth: 600 mm Height: (with lid closed) 550 mm Weight: approx. 57 kg Manufacturing country: Austria * Contact your supplier for additional information! ** Recommendation: Leica CV Ultra Instruction Manual V 1.1 - 11/2002...

  • Page 13: Initial Setup

    CV5030 are easily inflammable and hazardous to health. The installation The unit must be transported in upright posi- location of the CV5030 must be well- tion only as indicated by labels on the outside ventilated; there should be no igni- of the transport box.
  • Page 14 Initial setup • Fit the nozzle to the dispensing valve. • Fill prime container halfway with xylene or xylene substitute (according to the moun- tant used). • Insert the dispensing valve in paused posi- tion on the operating plate. • Plug the cable to the dispensing valve into the sleeve of the cover plate behind the operating plate.
  • Page 15 Fig. 6 The coverslip collection tray is hung on the right side on the slide ejector. It can be re- moved for cleaning purposes. Fig. 7 Leica CV5030 - Robotic Coverslipper...

  • Page 16: Transport Lock

    4. Initial setup 4.3. Transport lock Transport lock for coverslip transfer module Transport lock for the gripper Fig. 9 Fig. 8 Refilling consumables Use the glass bottles from the stan- dard delivery or suitable GLASS con- tainers – but never use PLASTIC con- tainers.

  • Page 17: Operating The Cv5030

    (holding the key pressed for 2 s) to set the coverslip parameters. The RELEASE COVER SLIP key releases the suction devices on the cov- erslip transfer arm (locked while the BUSY LED is lit), and the coverslip is released. Leica CV5030 - Robotic Coverslipper...
  • Page 18 5. Operating the CV5030 The RELEASE SLIDE key opens the gripper jaws as long as the key is pressed (blocked while the BUSY LED is lit). After extended non-operat- ing times, it may take several seconds until the gripper opens.

  • Page 19: Program Flow

    After initialization: priming required; extended warning signal if incor- rect key pressed (e.g. [START]); if ST5020 is connected, a regular 3s warning sequence will be triggered as the CV5030 is not prepared to accept a rack Dispenser located at P&P, i.e. not in the prime position (after startup or when [PRIME] is pressed;...
  • Page 20 [PAUSE] was pressed; this message appears while the coverslipping of the current slide is completed. CV5030 is paused; press [START] to continue the coverslipping pro- cess with parameter set n (in this example, 1). Coverslip magazine is empty; coverslipping operation interrupted (accompanied by continuous audible signal).

  • Page 21: User Menu

    Mountant type for the parameter set n (in this example, 1); N: 1-5; the parameter directly affects the bottle pressure; raising by one step means a pressure increase of 25%; • [START]/[PAUSE] sets the value • [RESPOND] ¡ Menu A main screen • [STANDBY] quits MENUs Leica CV5030 - Robotic Coverslipper...
  • Page 22 Operating the CV5030 Correction of the mountant stroke for the parameter set n (in this ex- ample, 1); NNN can be a value from -100 to 0 (in increments of 10), with -100 being a reduction by 10 mm; the total volume of mountant does not change, but is distributed over a shorter line from a different starting point;...
  • Page 23 • [RESPOND] ¡ MAGAZINE LIMIT main screen Error message if no coverslip found • [RESPOND] ¡ MAGAZINE LIMIT main screen From MAGAZINE LIMIT main screen: • [RESPOND] or [PAUSE] ¡ Menu B main screen • [STANDBY] ¡ quits MENUs Leica CV5030 - Robotic Coverslipper...

  • Page 24: Priming

    5. Operating the CV5030 Priming • Mountant can be let off into the prime con- Pressing the PRIME key opens the dispensing valve as long as the tainer at the paused position for the dis- key is pressed. If the operating pensing valve (do not overfill!).
  • Page 25 (A removal is not neces- sary if the remaining capacity is sufficient for the next coverslipping cycle.) If the output racks are raised to the extraction position using the LIFT key, all slides must be removed. Leica CV5030 - Robotic Coverslipper...

  • Page 26: Interrupting The Coverslipping Process

    Operating the CV5030 Interrupting the coverslipping process Press PAUSE to interrupt the coverslipping process. • The currently processed slide is completely coverslipped and pushed into the output rack. • The PAUSE LED lights up. • For extended non-operating times, place the dispensing valve in the paused position (prime container).

  • Page 27: Process-Based Interruptions

    - ERROR mode: Continuous alarm, display shows “CHECK COVER SLIPS”, - Acknowledge the audible alarm by pressing the RESPOND key, - Fill the coverslip magazine, - Continue the coverslipping process with START (after acknowl- edging the alarm). Leica CV5030 - Robotic Coverslipper...
  • Page 28 5. Operating the CV5030 • In secure mode (see section 5.4) - The processes are identical to those in quick mode, but since no slide has been removed from the bath, only a short alarm se- quence is triggered to indicate the error state.
  • Page 29 Recommended settings for media type, mountant volume and pressure 1. Histology Mountant Mountant Nozzle Coverslip size medium Stroke Volume Type Permount Pertex CV Mount CV Ultra 2. Cytology Mountant Mountant Nozzle Coverslip size medium Stroke Volume Type Permount Pertex CV Mount CV Ultra Leica CV5030 - Robotic Coverslipper...

  • Page 30: Operating The Cv5030 In Connection With The Ts5025 Transfer Station

    • Status changes to READY. • ST5020 recognizes if the CV5030 is switched off or not switched to standby mode (no feedback). If the CV5030 is not ready for operation, the racks are placed in the exit station of the ST5020 and must be transferred manually.

  • Page 31: Interruption By The User

    Operating the CV5030 in connection with the TS5025 transfer station • If CV5030 is activated but not ready for processing a new input rack, the ST5020 is prompted for a delayed request: • If priming is required: “PRIME”” display and audible signal.

  • Page 32: Process-Based Interruptions

    Operating the CV5030 in connection with the TS5025 transfer station The remaining slides can, therefore, be processed by pressing the START key. An empty slide rack must then be manually removed from the bath (using the door and pulling out the bath guide).

  • Page 33: Switching Off The Unit

    If solvents remain in the unit after it is de-energized, solvent va- pors can develop. If the unit is not operated using a vent, danger of fire and poisoning exits! * e.g. xylene or substitute – depending on the mountant used Leica CV5030 - Robotic Coverslipper...

  • Page 34: Cleaning And Maintenance

    Cleaning and maintenance Daily cleaning • After extended non-operating times and generally always before priming, the nozzle must be checked for dried residues of the mountant and cleaned, if necessary. • The suction devices for lifting coverslips must be regularly checked for damage and dirt accumulation.
  • Page 35 Check prime container, empty or fill, if necessary Replace activated carbon filter Check suction cups, clean or replace if necessary Clean gripper Clean bath Check coverslip magazine Clean loading door Clean complete working area Leica CV5030 - Robotic Coverslipper...

  • Page 36: System And Error Messages

    9. System and error messages • PRIME: Appears after activating the unit from standby mode and vent- ing the mountant bottle at the display. As long as this message is dis- played, all keys except PRIME, VENT MOUNTANT, STOP and STANDBY are locked.
  • Page 37 • ST5020 REQUEST: ST5020 maintains a slide rack for CV5030. But CV5030 is not capable of directly accepting (e.g. it may be busy pro- cessing another input rack); the message is generally accompanied by an additional message about the cause of the prevention. This prevention must be removed for the transfer to be successful (addi- tional request through ST5020 after 60 s).
  • Page 38 TS5025, but it could not be processed (due to user intervention, e.g. venting the mountant bottle) and is now waiting in the transfer posi- tion of TS5025. Remedy: Prepare the CV5030 for operation (push in the bath guide, prime the mountant bottle); this erases the display and the rack is processed.
  • Page 39 Transfer station: • Error 2 61 Transfer station Please contact our technical service. • Error 2 62 Transfer station Please contact our technical service. • Error 2 63 Transfer station Please contact our technical service. Leica CV5030 - Robotic Coverslipper...
  • Page 40 9. System and error messages Error type 3 - Device in operation (Busy LED green) Error message Area of problem Action From CPU version V1.02.00 • Error 3 11 Internal. communication error Reinitialize! or contact our technical service. • Error 3 12 Internal.
  • Page 41 • Error 4 62 Rack Reinitialize! or contact our technical service. • Error 4 63 Home position Reinitialize! or contact our technical service. • Error 4 64 Transfer station Reinitialize! or contact our technical service. Leica CV5030 - Robotic Coverslipper...

  • Page 42: Accessories

    - Leica ST Ultra was developed as a xylene replacement for histological specimen mounting, paraffin removal, as well as manual and automatic specimen mounting. For specimen mounting, ST Ultra can only be used in conjunction with Leica CV Ultra. 1 canister, 10 liters •...
  • Page 43 • Leica CV5030 - container for Medite/Hacker slide racks for 20 slides • Leica CV5030 - coverslip catch dish • Leica CV5030 - suction cups, pack of 2 • Leica CV5030 - dispenser holder mount • Leica CV5030 - demo packaging •...

  • Page 44: Warranty And Service

    11. Warranty and service Warranty Leica Microsystems Nussloch GmbH vouches for the fact that the deliv- ered product underwent comprehensive quality control based on its strict internal testing criteria and is free of defects, and guarantees that all technical specifications and/or warranty of qualities was met.

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