Determing The Optimum Parameter Setting (Menu A+B); Procedure - Leica CV5030 Instructions For Use Manual

5
Operation
Designation
Consul-Mount
/ Cytology
Formulation
Shandon
Xylene
Substitute
Mountant
Histokitt II
Aquatex:
(water based)

5.13 Determing the optimum parameter setting (MENU A+B)

L The following procedure provides assistance for:
• Adjusting the previously listed parameter setting to onsite lab conditions.
• Determining the optimum parameter settings for an unlisted coverslip mountant
Recommendation for parameter setting (beginning with firmware 3.01.04)).
• Changing from coverslipping with solvent ("wet") to coverslipping without solvent ("dry") in the loading
bath, or, reversed
The following sections give a detailed description of the steps to carry out, from the instrument
preparation through to specimen evaluation. During the test series for determining the optimum
parameter sets, the coverslipping operation in the instrument should also be observed.

5.13.1 Procedure

1. Filling the coverslip mountant
• It is imperative that there be no air bubbles in the coverslip mountant during parameter setting (use
clean bottles; (→ p. 38 – 4.11 Refilling consumables)).
2. Selecting the suitable size or number of dispenser needles (nozzles)
The following are suitable for high-viscosity coverslip mountants:
• Dispenser needle No. 16 (largest diameter),
• Dispenser needle No. 18
68
Manufacturer/ Loading bath
supplier filled with
Shandon/Thermo Xylene
Scientific
Shandon/Thermo Xylene
Scientific
Carl Roth GmbH Xylene
Merck KGaA Distilled water
(→ p. 69 – 3. Differences between "wet" and "dry"
Dispenser STK VOL
needles
18 50 4/5
20 50 4/5
20 50
20 50 7/8
coverslipping).
Type LEN
6 0
5 0
6 7 0
7/8 STP:
40/50
CSP:
varies
(→ p. 65 – 5.12
Version 3.4, Revision M