Leica CV5030 Instructions For Use Manual page 71

The parameters VOL
application quantity on the specimen slide is attained. Further procedures are described in the following
Point (→ p. 71 – 8. Fine-tuning the adhesive and coverslip support
7c. Guideline for high-viscosity coverslip mountant
Dispenser needle: 18 or 16 (largest diameter)
• STK: Adjust stroke length to the cover slip length used (e.g.
and 24 x 50 mm).
• VOL: Start the first coverslip trials with VOL
• TYP: Start the first coverslip trials with the setting TYP
The parameters VOL
application quantity on the specimen slide is attained. Further procedures are described in the following
Point (→ p. 71 – 8. Fine-tuning the adhesive and coverslip support
Note
Important note:
If TYP is at the maximum setting (10 - highest pressure), it is possible to generate small air bubbles in
the dispenser system. This depends on specific properties of the respective coverslip mountant and
the selection of dispenser needle (frequently occurring with small-diameter needles). Therefore it is
advisable to begin with the mid-range parameter settings when determining the optimum parameter
sets.
Unsuitable procedures:
Dispenser needle No. 21
TYP: 10
VOL: 1 or 2 incremental increase.
Suitable procedures:
Dispenser needle No. 21
TYP: 4 or 5 incremental increase/reduction, with parallel incremental increase/reduction of
VOL: 4 or 5 incremental increase/reduction, with parallel incremental increase/reduction of
If a suitable coverslip mountant application quantity cannot be attained, switch to a dispenser needle
with a different diameter.
8. Fine-tuning the adhesive and coverslip support positioning
Here, the following parameters must be matched to each other:
STP (Stroke position - starting point for the coverslip mountant stroke)
CSP (Cover slip placement position) and
LEN (Stroke length correction for the coverslip mountant stroke)
Leica CV5030
and TYP are incrementally adjusted (increase or reduction) until a suitable
and TYP are incrementally adjusted (increase or reduction) until a suitable
positioning).
MENU A:
STK = 50 for coverslip size 22 x 50 mm
(approx. 5-7)
(approx. 5-7)
positioning).
5
Operation
VOL
TYP
71