Leica CV5030 Instructions For Use Manual page 70

5
Operation
5. Important information regarding test run procedure for determining the optimum coverslipping
parameters
Note
• The initial coverslipping trials should be carried out with empty specimen slides, i.e. without a
specimen.
• If coverslipping quality is good, subsequently carry out a test series using an applied specimen.
• Since factors such as section thickness and specimen composition and type can affect the
coverslipping quality, it may be necessary to make minor corrections to the parameter settings.
Ex. VOL: Increase or reduction of adjustment by ± 1
and/or TYP: Increase or reduction of adjustment by ± 1
6. Evaluation of coverslipping quality
The respective coverslipping quality of the test runs should be checked at various points in time for
adhesive and air bubbles:
• Directly after coverslipping operation.
• 1 - 3 hours after coverslipping.
• 24 - 48 hours after coverslipping.
Should air pockets appear between the specimen slide and cover slip after these checks, the settings
must be corrected.
If air pockets still occur despite suitable changes,
directions.
If artifacts become visible within the monitoring process (streaks, cloudiness, discoloration), the involved
reagents must be inspected for compatibility and storage life.
The staining protocol should be adapted as needed.
7. Inputting the first test parameters
7a. Effect of parameters on the application quantity
• STK - Stroke length of the coverslip mountant corresponding to the coverslip length used.
• VOL - Quantity of the coverslip mountant: 1 (min.) -> 9 (max.)
• TYP - Pressure in the mountant bottle: 1 (min.) -> 10 (max.)
7b. Guideline for low-viscosity coverslip mountants
Dispenser needle: 20 or 21 (smallest diameter)
• STK: Adjust stroke length to the cover slip length used (e.g.
and 24 x 50 mm).
• VOL: Start the first coverslip trials with VOL
• TYP: Start the first coverslip trials with TYP
70
(→ p. 93 – 8.2 Troubleshooting)
MENU A:
STK = 50 for coverslip size 22 x 50 mm
set low (approx. 2-4)
set low (approx. 2-4)
provides additional
Version 3.4, Revision M