Qiagen QIAcuity 911000 User Manual page 171

Gene expression
The Software Suite analyzes the gene expression of the samples. The analysis results are put into
list views, heatmaps, point diagrams, and concentration diagrams
Note: Saving gene expression tests is not yet provided.
Setting up a gene expression analysis
1. Click the applicable wells in the plate layout.
2. Select the applicable reference sample from the Reference sample list.
3. Select the applicable target from the Target of interest list.
4. Select one or more reference targets from the Reference target(s) list.
5. To view the results of the analysis, click Show results.
The results are divided into several tabs. To view the contents of the tab, click the tab title.
List tab for gene expression
The list tab contains a table with an overview of the analyzed wells. The following columns are
available in the table:
Well ID – This column represents the well position in the plate layout.
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Sample/NTC/Control Name – This column shows the sample, NTC, or control name with its

corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control. Reference samples are marked with the word Ref.
Reaction mix – This column contains the icon and the name of the Reaction mix.

Target – This column shows all target names with its corresponding target type. Targets which

were selected as target of interest (TOI) or reference target (Ref) are marked accordingly.
Concentration (copies/ul) – This column shows the concentration assigned to each target.
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CI (95%) - This column shows the value of the confidence interval at a 95% confidence level.
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Fold change – This column shows the change in the level of gene expression in the sample.

CI (95%) – This column shows the value of the confidence interval for the fold change at a

95% confidence interval.
Fold regulation – This column shows the change in the level of gene expression compared to
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the reference sample.
QIAcuity User Manual 07/2020 171