Qiagen QIAcuity 911000 User Manual page 150

2D Scatterplot – Lasso mode – Group selection before free-hand assignment.
Use the Auto-threshold button for each axis to re-set the threshold to the values, which is calculated
by the analysis algorithm for each well. Click the Recalculate button to trigger the re-analysis of
data.
If you change Common thresholds or the assignment of partitions using the Lasso mode and wants
to leave the 2D Scatterplot view without triggering the Recalculate button a propriate warning
message windows opens. You can continue ignoring your changes or stay in the 2D Scatterplot
view.
To add the 2D scatterplot to the report, click Add to report. For more information on reports, see .
Concentration diagram tab for absolute quantification
The Concentration Diagram tab shows the diagrams that display the distribution of concentration
values and confidence intervals. One diagram is created for each selected target or channel. A
concentration diagram has two axes. The x-axis shows the analyzed wells, and the y-axis represents
the concentration values for the selected targets or channels of each well. The values on the y-axis
have two available scales – linear and logarithmic.
To view an additional toolbar that enables you to perform actions related to the diagram, such as
downloading the plot, hold the pointer over the diagram. For more details about the toolbar, refer
to section Diagram options
The y-axis scale can be modified using the buttons located below each graph. The buttons are
visible when you hold the pointer over a graph. To view the values on a linear scale, click lin. To
view the values on a logarithmic scale, click log10.
Each diagram presents two values for each well – the concentration value, displayed as a bar, and
the confidence interval, displayed as an error bar. To view the exact values, point to one of the
QIAcuity User Manual 07/2020 150