Qiagen QIAcuity 911000 User Manual page 169

Concentration diagram tab for copy number variation
The concentration diagram tab shows the diagram that displays the concentration values of the
configured copy number variation test and the confidence intervals related to every value. A
concentration diagram has two axes.
x-axis represents labels of wells and samples that it belongs to

y-axis represents the concentration values for selected targets or channels for each well. A

confidence interval displayed as an error bar is shown for each of the bars on the diagram.
The y-axis scale can be modified using the buttons located below each graph. The buttons are
visible when you hold the pointer over a graph. To view the values on a linear scale, click lin. To
view the values on a logarithmic scale, click log10.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to Diagram
options
The Concentration diagram is a bar plot which presents two values:
Concentration value as bar

CI value as error bar

The diagram consists of all possible combinations. Each sample on the diagram is represented by
the number of bars according to targets that was selected as a target of interest and reference
targets. Samples on the diagram are sorted by the sample ID. Target bars in the sample show one
concentration bar for target of interest at fist position and concentration bars for reference targets
sorted by their well id. The bar color reflects the color assigned to the corresponding targets. The
sample ID is shown center-aligned below the bars.
The Bar size is fixed if:
The reference targets and target of interests are situated on one well there is one well label for

all that belongs to the same well.
The reference targets and target of interests are situated on two wells or more wells there are

one well label per targets that are within this well.
When hovering over a bar in the diagram, a tooltip appears presenting details about the actual
values of concentration and CI and results of tests.
QIAcuity User Manual 07/2020 169