Qiagen BLU-V User Manual
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Qiagen BLU-V User Manual

For neutralization of dna of dead organisms
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September 2016
BLU-V
System User Manual
®
For neutralization of DNA of dead organisms
Sample & Assay Technologies

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Summary of Contents for Qiagen BLU-V

  • Page 1 September 2016 BLU-V System User Manual ® For neutralization of DNA of dead organisms Sample & Assay Technologies...
  • Page 2 QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Our advanced, high-quality products and services ensure success from sample to result. QIAGEN sets standards in: Purification of DNA, RNA, and proteins ...

  • Page 3: Table Of Contents

    Contents Contents Introduction 1.1 Intended Use of the BLU-V system 1.2 General Information Safety Information 2.1 Proper use 2.2 Electrical safety 2.3 Environment 2.4 Biological safety 2.5 Chemicals 2.6 Safety maintenance 2.7 Waste disposal 2.8 Symbols on the BLU-V System General Description 3.1 Features of the BLU-V System...
  • Page 4 Contents Warranty Appendix B: Declaration of Conformity Appendix C: BLU-V System Accessories Appendix D: Related Products BLU-V User System Manual 09/2016...

  • Page 5: Introduction

    Thank you for choosing the BLU-V System. We are confident that this instrument will become an integral part of your laboratory. Before using the BLU-V System, it is essential that you read this user manual carefully. Following the instructions and safety information in this user manual will ensure safe operation and maintain the system in a safe condition.
  • Page 6 Introduction difficulties regarding the BLU-V System or QIAGEN products in general, do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists, as well as to the researchers at QIAGEN.

  • Page 7: Safety Information

    Safety Information 2. Safety Information Before using the BLU-V System, it is essential that you read this user manual carefully. Following the instructions and safety information in this user manual will ensure safe operation and maintain the system in a safe condition.
  • Page 8 Standard-Sicherheitsanforderungen die im jeweiligen Land vorherrschen. Les conseils donnés dans ce manuel ont pour but de venir compléter les exigences de sécurité habituelles en vigueur dans le pays de l’utilisateur et non de s’y substituer. BLU-V User System Manual 09/2016...

  • Page 9: Proper Use

    Risque de dommages corporels et matériels L’utilisation non convenable du BLU-V System peut causer des blessures ou des détériorations de l’instrument. Le BLU-V System ne doit être utilisé que par du personnel qualifié qui a été formé de façon appropriée. CAUTION...

  • Page 10: Electrical Safety

    Chemikalien verursachte Geräteschäden sind nicht durch die Garantie abgedeckt. Déterioration de l’appareil Eviter de renverser de l’eau ou des substances chimiques sur le BLU-V System. Tout dommage causé par de l’eau ou des produits chimiques mettra fin à la garantie.
  • Page 11 Safety Information Si le fonctionnement du BLU-V system est interrompu pour quelque raison que ce soit (coupure d’alimentation ou problème mechanique), éteignez le BLU-V system à l’aide de l’interrupteur d’alimentation puis débranchez le câble d’alimentation de la prise de courant. Contactez le Service Technique de QIAGEN si un incident de ce type survient.
  • Page 12 Safety Information To ensure satisfactory and safe operation of the BLU-V System: The line power cord must be connected to a line power  outlet that has a protective conductor (earth/ground). The instrument must not be operated with the cover ...

  • Page 13: Environment

    2.3 Environment Operating conditions WARNING Explosive atmosphere [W3] The BLU-V System is not designed for use in an explosive atmosphere. Explosionsfähige Atmosphären Das BLU-V System darf nicht in explosionsfähigen Atmosphären betrieben werden. Atmosphère explosive Le BLU-V System n’est pas conçu pour fonctionner dans une atmosphère explosive.

  • Page 14: Biological Safety

    Laisser un espace d’au moins 10 cm sur les côtés et à l’arrière du BLU-V System pour assurer une ventilation efficace. Les grilles et prises d’air assurant la ventilation du BLU-V System ne doivent pas être couvertes. 2.4 Biological safety...
  • Page 15 Safety Information WARNING Samples containing infectious agents [W5] Some samples used with the BLU-V System may contain infectious agents. Handle such samples in accordance with the required safety regulations. The responsible person(s) (e.g., laboratory manager) must take the necessary precautions to ensure that the workplace is safe and that the...
  • Page 16 - concernant la santé et la sécurité. *OSHA: Occupational Safety and Health Administration (United States of America). † ACGIH: American Conference of Government Industrial Hygienists (United States of America). COSHH: Control of Substances Hazardous to Health (United Kingdom). ‡ BLU-V User System Manual 09/2016...

  • Page 17: Chemicals

    WARNING Hazardous chemicals [W6] Some chemicals used with the BLU-V System may be hazardous or may become hazardous after completion of the protocol run. Always wear safety glasses, gloves, and a lab coat. The responsible person(s) (e.g., laboratory manager) must take the...
  • Page 18 - concernant la santé et la sécurité. *OSHA: Occupational Safety and Health Administration (United States of America). † ACGIH: American Conference of Government Industrial Hygienists (United States of America). COSHH: Control of Substances Hazardous to Health (United Kingdom). ‡ BLU-V User System Manual 09/2016...

  • Page 19: Safety Maintenance

    Damage to the instrument [C3] Do not use spray bottles containing alcohol or disinfectant to clean the surface of the BLU-V System instrument or its parts. Spray bottles should be used only to clean items that have been removed from the instrument.

  • Page 20: Waste Disposal

    Les consommables et flacons utilisés peuvent contenir des agents chimiques ou infectieux dangereux. Ces déchets doivent être convenablement collectés et mis au rebut conformément aux règles de sécurité locales. Pour savoir comment mettre au rebut le BLU-V system, voir l'annexe A. BLU-V User System Manual 09/2016...

  • Page 21: Symbols On The Blu-V System

    Safety Information 2.8 Symbols on the BLU-V System Symbol Location Language Description Type plate on the Serial number bottom of the instrument Plakette auf der Seriennummer Unterseite des Gerätes Plaque au-dessous de Numéro de série l'appareil Type plate on the...
  • Page 22 équipements électriques et électroniques) Type plate on the Consult instructions for use bottom of the instrument Plakette auf der Beachten Sie die Unterseite des Gerätes Anwendungshinweise Plaque au-dessous de Consulter le mode d’emploi l'appareil BLU-V User System Manual 09/2016...

  • Page 23: General Description

    General Description 3. General Description The BLU-V System is a thermally stable instrument that generates a constant and uniform light dosage to allow simultaneous photo-activation of 12 samples in a simple and efficient manner. Its design minimizes intra-and inter-laboratory variability.
  • Page 24 Note: You may only perform a run when the protection shield is closed. A sensor detects whether the shield is closed or not. 3.1.3 Air inlet fan The air inlet fan is located on the right side of the instrument. BLU-V User System Manual 09/2016...
  • Page 25 ON/OFF switch Note: Details on changing the fuse are given in the “Maintenance” section. 3.1.5 Power cable The BLU-V System is equipped with a power cable with a plug suitable for the destination country. 3.1.6 BLU-V Sample Holder The BLU-V Sample Holder enables the insertion of 12 sample tubes, each with a tube volume of up to 2 ml into the BLU-V System instrument.
  • Page 26 The BLU-V Incubation Box is intended for light-protected incubation of reaction solutions containing photo-reactive reagents. The BLU-V Incubation Box and a second BLU-V Sample Holder for use with it must be ordered separately. For details, see Appendix B: BLU-V System Accessories.

  • Page 27: Installation

    4. The green LED beside the start button on the control panel flashes green when the instrument is ON. When the BLU-V System is not in use for a longer period of time, we recommend disconnecting the power cable. BLU-V User System Manual 09/2016...

  • Page 28: Grounding Requirements

    AC power cord. To preserve this protection feature, do not operate the instrument from an AC power outlet that has no ground (earth) connection. For replacement of the 3-conductor AC power cord, contact QIAGEN Technical Services for an authorized spare part.

  • Page 29: Operating Procedures

    1. Prepare the reaction solutions. 2. Insert the reaction tubes into the BLU-V Sample Holder. 3. Place the BLU-V Sample Holder with the sample tubes into the BLU-V System. Note: If the BLU-V Incubation Box with the BLU-V Sample Holder has been used, this holder can be directly transferred into the instrument.
  • Page 30 Laisser un espace d’au moins 10 cm sur les côtés et à l’arrière du BLU-V System pour assurer une ventilation efficace. Les grilles et prises d’air assurant la ventilation du BLU-V System ne doivent pas être couvertes. 5.1.1 Setting the time The display shows the selected time in minutes.

  • Page 31: Performing The Run

    Adjust the light intensity settings based on the recommendations given in  the corresponding BLU-V Kit handbooks. For standard illumination settings keep the light power at 100%. Note: The setting of the last completed run is saved as the new default.
  • Page 32 4. After 30 seconds the indicator LED will change from red to flashing green and the set time is displayed again, open the protective shield and remove the BLU-V Sample Holder with the sample tubes. 5. Keep the instrument on until the fan stops. This helps to cool the instrument.

  • Page 33: Maintenance

    Maintenance 6. Maintenance 6.1 Cleaning procedure Disconnect the BLU-V System from the power supply for cleaning. Use a lightly moistened cloth or an antistatic cloth. Do not use cleaning agents or abrasive solvent products. CAUTION Damage to the instrument [C3] Do not use spray bottles containing alcohol or disinfectant to clean the surface of the BLU-V System instrument or its parts.
  • Page 34 4. Remove the fuse (indicated by the red arrow B) from the fuse holder. 5. Insert the new fuse and insert the fuse holder into the instrument. Specifications of the fuse are given in Appendix A “Technical data”. BLU-V User System Manual 09/2016...

  • Page 35: Technical Data

    Appendix Appendix A Technical Data QIAGEN reserves the right to change specifications at any time. Environmental conditions Operating conditions Power range 100–240 V AC Mains supply voltage fluctuations must not exceed +/- 10% of nominal supply voltages Frequency range 50/60 Hz...

  • Page 36: Waste Electrical And Electronic Equipment

    Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE-marked electronic equipment is provided.
  • Page 37 Consult the dealer or an experienced radio/T.V. technician for help.  QIAGEN GmbH Germany is not responsible for any radio television interference caused by unauthorized modifications of this equipment or the substitution or attachment of connection cables and equipment other than those specified by QIAGEN GmbH, Germany.
  • Page 38 Appendix Warranty The BLU-V System instrument has a 24-month warranty period which starts when the final customer purchases it. The warranty is void when misuse of the equipment can be proved. Damage or manufacturing faults caused by impacts, chemical or corrosive products, liquids, damp, or other external factors, such as radiation, fire, or inadequate transport are not included.
  • Page 39 PMA Kit Buffer, RNAse-Free water For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services. BLU-V User System Manual 09/2016...
  • Page 40 Trademarks: QIAGEN , Sample to Insight , BLU-V (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. HB-1761-006 © 2014–2016 QIAGEN, all rights reserved.
  • Page 41 Notes BLU-V User System Manual 09/2016...
  • Page 42 Notes BLU-V User System Manual 09/2016...
  • Page 43 Notes BLU-V User System Manual 09/2016...
  • Page 44 Australia techservice-au@qiagen.com  Austria techservice-at@qiagen.com  Belgium techservice-bnl@qiagen.com  Brazil suportetecnico.brasil@qiagen.com  Canada techservice-ca@qiagen.com  China techservice-cn@qiagen.com  Denmark techservice-nordic@qiagen.com  Finland techservice-nordic@qiagen.com  France techservice-fr@qiagen.com  Germany techservice-de@qiagen.com  Hong Kong techservice-hk@qiagen.com  India techservice-india@qiagen.com  techservice-uk@qiagen.com Ireland ...

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