Qiagen QIAcuity 911000 User Manual page 136

On the right side above the table there is a checkbox which allows the user to show mean values
for replicates in addition. By default, the button is unchecked, and results are displayed without
mean values. When the button is checked the list still shows independent rows for each selected
well, but replicates are grouped together. Replicates from the same group are next to each other in
the list view. There are two additional columns indicating the mean values:
Mean concentration
CI (95%) - CI of mean concentration
List tab with selected mean values for replicates.
Signalmap tab for absolute quantification
The Signalmap tab provides positive partitions for target channels and valid partitions for the
reference channel of selected wells. For each channel included in target or channel selection, one
signal map view is created. The signal map views are sorted by channel position in imaging
("Green", "Yellow", "Orange", "Red", "Crimson"), separated by a horizontal line.
With this view the user can visually check the influence of threshold settings on the partition
distribution and consider the impact of artefacts that he might have discovered in the source image
view.
Each signal map represents the plate layout for a selected channel where only the images of the
selected wells are loaded. Remaining wells are displayed as grey squares. When the image of a
well could not be calculated by the algorithm, a placeholder image is shown. On hovering the
mouse over the well, the user is informed that the signal map for this well could not be created.
QIAcuity User Manual 07/2020 136